FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Aos1c06048
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General Information
Symbol
Dmel\Aos1c06048
Species
D. melanogaster
Name
FlyBase ID
FBal0162805
Feature type
allele
Associated gene
Dmel\Aos1
Associated Insertion(s)
PBac{PB}Aos1c06048
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Kanakousaki and Gibson, 2012)
Mutagen
piggyBac activity
(Bloomington Drosophila Stock Center, 2004.11.6)
Progenitor genotype
Associated Insertion(s)
PBac{PB}Aos1c06048
Cytology
Description

Insertion in the 5' UTR, 41bp upstream of the ATG codon.

(Kanakousaki and Gibson, 2012)
Allele components
Component
Use(s)
Also carries
FRT
Mutations Mapped to the Genome
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  hematologic cancer
      CEA
      (Kalamarz et al., 2012)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous third instar larvae occasionally have melanotic tumours. Homozygotes show developmental delay (puparium formation is delayed relative to wild type) and highly penetrant early pupal lethality. Imaginal discs are missing in homozygous larvae.

      Aos1c06048/Df(3R)ED5554 and Aos1c06048/Df(3R)ED5591 animals show loss of imaginal discs and pupal lethality.

      Homozygous clones in the wing disc are small, fragmented and show high levels of cell death 3 days after clone induction. The defects in growth and viability of homozygous clones are not rescued if the clones are induced in a Minute background.

      Cells in homozygous clones in the imaginal discs accumulate in G2/M of the cell cycle. EdU incorporation in the salivary glands of mutant early third instar larvae appears normal.

      (Kanakousaki and Gibson, 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4Act.PU in homozygous Aos1c06048 clones in the wing disc suppresses the increased cell death seen in these clones, but does not rescue the growth defect, as the double mutant clones are still smaller in size than control clones. The rescued cells appear significantly larger than control cells are are partially extruded from the epithelial layer, leaving only one or two thin membraneous processes attached to the apical epithelial surface.

      (Kanakousaki and Gibson, 2012)
      Complementation and Rescue Data
      Rescued by

      Aos1c06048 is rescued by Aos1+t2.9

      (Kanakousaki and Gibson, 2012)

      Aos1c06048 is rescued by Aos1UAS.cKa/Scer\GAL4Act.PU

      (Kanakousaki and Gibson, 2012)
      Comments

      Expression of Aos1Scer\UAS.cKa under the control of Scer\GAL4Act.PU in the clone rescues the cell lethality of Aos1c06048 clones.

      (Kanakousaki and Gibson, 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Precise excision of the insertion reverts the mutant phenotype.

      (Kanakousaki and Gibson, 2012)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Aos1c06048
      (Kalamarz et al., 2012)
      aos1c06048
      (Gao et al., 2024, Kanakousaki and Gibson, 2012)
      Name Synonyms
      Secondary FlyBase IDs
        References (6)