FlyBase Allele Report: Dmel\Zwilch[1229]

General Information

Symbol

Dmel\Zwilch¹²²⁹

Species

D. melanogaster

Name

FlyBase ID

FBal0177077

Feature type

allele

Associated gene

Dmel\Zwilch

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Williams et al., 2003)

Mutagen

ethyl methanesulfonate

(Williams et al., 2003)

Progenitor genotype

Cytology

Description

Contains a nonsense mutation that would shorten any translated protein from 644 to 603 amino acid residues.

(Williams et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:31,211,361..31,211,361 [-]

Nucleotide change:

C31211361A

Amino acid change:

S604term | Zwilch-PA

Reported amino acid change:

?604term

Comment:

Zwilch¹²²⁹ is reported as a nonsense mutation that shortens the protein to 603 amino acids.

(Williams et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle

(Williams et al., 2003)

abnormal mitotic cell cycle

(Williams et al., 2003)

female sterile | recessive

(Williams et al., 2003)

Phenotype Manifest In

anaphase & condensed nuclear chromosome

(Williams et al., 2003)

Detailed Description

Statement

Reference

Mutant spermatocytes have a normal number of Golgi structures but show a mild defect in shape.

Spermatids from mutant third instar larvae testes do not show cytokinesis defects.

(Wainman et al., 2012)

The brains of homozygous zwilch¹²²⁹ larvae have many aneuploid cells. Some zwilch¹²²⁹ larval brain cells in anaphase show aberrant chromosomes that lag at the metaphase plate, while others show an unequal distribution of chromosomes between the two spindle poles. However, chromosome congression to the metaphase plate and the morphology of both the spindle and chromosomes appears normal. Unlike wild-type cells, zwilch¹²²⁹ mutant cells are not arrested in metaphase in response to treatment with colchicine, suggesting that the mutants have a failure of the spindle checkpoint. Meiosis is also affected in zwilch¹²²⁹ mutants, as zwilch¹²²⁹ testes show high levels of chromosome missegregation during both meiotic divisions.

(Williams et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Zwilch¹²²⁹/Zwilch¹²²⁹ is a suppressor | partially of abnormal mitotic cell cycle phenotype of nudE^39A

(Wainman et al., 2009)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The mitotic index and the proportion of mitotic figures in anaphase relative to those in prometaphase or metaphase is nearly normal in the brains of nudE^39A zwilch¹²²⁹ double homozygous larvae.

(Wainman et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Zwilch¹²²⁹

zwilch¹²²⁹

(Wainman et al., 2012, Wainman et al., 2009)

zwilch^mit-1229

(Williams et al., 2003)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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