FlyBase Allele Report: Dmel\wun2[N14]

General Information

Symbol

Dmel\wun2^N14

Species

D. melanogaster

Name

FlyBase ID

FBal0177109

Feature type

allele

Associated gene

Dmel\wun2

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Hanyu-Nakamura et al., 2004)

Progenitor genotype

Cytology

Description

Amino acid replacement: W111term.

(Hanyu-Nakamura et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:9,416,675..9,416,675 [+]

Nucleotide change:

G9416675A

Amino acid change:

W111term | wun2-PA; W7term | wun2-PB

Reported amino acid change:

W111term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

(Hanyu-Nakamura et al., 2004)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

germline cell | embryonic stage 15 | germline clone | maternal effect

(Renault et al., 2010)

germline cell | embryonic stage 15 | maternal effect (with Df(2R)w45-19g)

(Renault et al., 2010)

germline cell | embryonic stage 16 | germline clone | maternal effect

(Renault et al., 2010)

germline cell | embryonic stage 16 | maternal effect (with Df(2R)w45-19g)

(Renault et al., 2010)

ovary & germ cell & adult | non-rescuable maternal effect (with Df(2R)w45-19g)

(Hanyu-Nakamura et al., 2004)

pole cell & gonad & embryo | non-rescuable maternal effect (with Df(2R)w45-19g)

(Hanyu-Nakamura et al., 2004)

primordial germ cell | embryonic stage | maternal effect

(Tarayrah-Ibraheim et al., 2021)

primordial germ cell | embryonic stage | non-rescuable maternal effect (with Df(2R)w45-19g)

(Hanyu-Nakamura et al., 2004)

Detailed Description

Statement

Reference

Embryos derived from wun2^N14/Df(2R)w45-19g females and wild type males have approximately 2 germ cells in stage 15/16 embryos (compared to approximately 21 in wild type).

Embryos derived from females carrying wun2^N14 germline clones and wild type males contain only one germ cell on average in stage 15/16 embryos (compared to approximately 20 in wild type).

(Renault et al., 2010)

Embryos from wun2^N14/Df(2R)w45-19g mothers form normal numbers of pole cells which migrate normally up to stage 10. However, by stage 11, the numbers of poles cells are dramatically reduced. Any remaining pole cells remain associated with the surface of the midgut, and in subsequent development, few or no pole cells are incorporated into the gonads. These animals develop into morphologically normal animals. However, 80% of the resulting adult females are agametic.

(Hanyu-Nakamura et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

DNaseII^lo, wun2^N14 has decreased cell number | maternal effect | embryonic stage phenotype

(Tarayrah-Ibraheim et al., 2021)

Phenotype Manifest In

Suppressed by

Statement

Reference

Df(2R)w45-19g/wun2^N14 has primordial germ cell | non-rescuable maternal effect | embryonic stage phenotype, suppressible | partially by Df(2R)w45-19g/wun^CE

(Hanyu-Nakamura et al., 2004)

Other

Statement

Reference

DNaseII^lo, wun2^N14 has primordial germ cell | maternal effect | embryonic stage | decreased number phenotype

(Tarayrah-Ibraheim et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

The pole cell death phenotype of embryos from wun2^N14/Df(2R)w45-19g mothers is partially suppressed by zygotic wun^CE/Df(2R)w45-19g, although proper migration of the pole cells is not rescued in these embryos (Note wun^CE/Df(2R)w45-19g vs wun^CE/+ embryos not indpendently scoreable, but in a 1:1 population of each, 50% had a less severe cell death phenotype than wun⁺ embryos from wun2^N14/Df(2R)w45-19g mothers. These were assumed to be wun^CE/Df(2R)w45-19g rather than wun^CE/+).

(Hanyu-Nakamura et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

wun2^N14 is rescued by wun2^+t17

(Hanyu-Nakamura et al., 2004)

wun2^N14 is rescued by wun2^+t8

(Hanyu-Nakamura et al., 2004)

Partially rescued by

Df(2R)w45-19g/wun2^N14 is partially rescued by Scer\GAL4^{VP16.nanos.UTR}/wun2^EP2650

(Hanyu-Nakamura et al., 2004)

Not rescued by

wun2^N14 is not rescued by wun2^t8.H326K

(Hanyu-Nakamura et al., 2004)

wun2^N14 is not rescued by wun2^t8.Δ

(Hanyu-Nakamura et al., 2004)

wun2^N14 is not rescued by wun2^t8.H274K

(Hanyu-Nakamura et al., 2004)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Isolated in an EMS screen to identify maternal mutations causing defects in pole cell development.

(Hanyu-Nakamura et al., 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

wun2^N14

(Chen et al., 2024, Tarayrah-Ibraheim et al., 2021, LeBlanc and Lehmann, 2017, Renault et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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