FlyBase Allele Report: Dmel\ds[UASp.cSa]

General Information

Symbol

Dmel\ds^UASp.cSa

Species

D. melanogaster

Name

FlyBase ID

FBal0180100

Feature type

allele

Associated gene

Dmel\ds

Associated Insertion(s)

Carried in Construct

P{UASp-ds.S}

Also Known As

UAS-Ds

Key Links

Transgenic product class

genomic_DNA

(Simon, 2004)

wild_type

(Simon, 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

genomic_DNA

(Simon, 2004)

wild_type

(Simon, 2004)

Mutagen

in vitro construct

(Simon, 2004)

Progenitor genotype

Carried in construct

P{UASp-ds.S}

Cytology

Description

UASp regulatory sequences drive expression of a genomic fragment extending from 35bp before the ds translational initiation codon to the end of exon 2 linked in frame to a genomic fragment containing the remaining ds coding exons and extending 1933bp beyond the termination codon.

(Simon, 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity, with Scer\GAL4^fj-VG1

(Simon, 2004)

Phenotype Manifest In

eye equator, with Scer\GAL4^fj-VG1

(Simon, 2004)

ommatidium, with Scer\GAL4^fj-VG1

(Simon, 2004)

Detailed Description

Statement

Reference

Flies expressing ds^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^αTub84B.PL in a ds^38k/ds^UAO71 background have a normal overall pattern of polarisation in the eye; an equator can easily be discerned. 97.6% of ommatidia are correctly constructed and rotated in these animals. Expression of ds^{Scer\UAS.P\T.cSa} under the control of either Scer\GAL4^αTub84B.PL or Scer\GAL4^bi-omb3-GAL4 has little effect on ommatidial polarity. Expression of ds^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^fj-VG1 often results in the equator region of ommatidia having reversed polarity. Expression of ds^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^fj-VG1 in a ds^38k/ds^UAO71 background results in eyes with disorganised ommatidial polarity.

(Simon, 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Flies expressing both fj^Scer\UAS.cZa and ds^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^αTub84B.PL in a ds^38k fj^N7/ds^UAO71 fj^d1 background have a disorganised ommatidial polarity; no obvious equator is present. Flies expressing ds^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^αTub84B.PL in a ds^38k fj^N7/ds^UAO71 fj^d1 background have a disorganised ommatidial polarity; no obvious equator is present. Flies expressing ds^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^fj-VG1 in a ds^UAO71 fj^VG1/ds^38k fj^N7 background have a disorganised ommatidial polarity; the eye contains a clearly perceptible equator in which most of the nearby ommatidia are reversed in polarity and thus point away from the equator. Flies expressing both fj^Scer\UAS.cZa and ds^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^αTub84B.PL in a ds^38k fj^N7/ds^UAO71 fj^d1 background have normal polarity throughout the wing blade with the exception of a small region along the anterior proximal margin of the wing, where a small swirl is often present. Flies expressing ds^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^αTub84B.PL in a ds^38k fj^N7/ds^UAO71 fj^d1 background have normal polarity throughout the wing blade with the exception of a small region along the anterior proximal margin of the wing, where a small swirl is often present.

(Simon, 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ds^{Scer\UAS.P\T.cSa}

ds^UASp.cSa

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

Open Close