UASt regulatory sequences drive expression of wild-type brk that is tagged at the C-terminal end with two copies of Tag:HA.
decreased size | adult stage, with Scer\GAL4nub-AC-62, Scer\GAL80elav.PU
decreased size | larval stage, with Hsap\RELAp65(AD)::pMag.Ubi, Scer\GAL4rn-G4DBD::nMagHigh1
lethal, with Scer\GAL4en-e16E
visible, with Scer\GAL4C-765
visible | adult stage, with Scer\GAL4nub-AC-62, Scer\GAL80elav.PU
wing, with Scer\GAL4nub-AC-62, Scer\GAL80elav.PU
wing blade, with Scer\GAL4C-765
wing disc | larval stage, with Hsap\RELAp65(AD)::pMag.Ubi, Scer\GAL4rn-G4DBD::nMagHigh1
Expression of brkScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4C-765 results in almost complete loss of the wing blade at 20oC.