The bi N, org-1 T and org-1 C domains have been fused in order separated by two or three junctional amino acids (A,G or T). Tag:MYC tags are at the C and N termini. A Tag:HA tag has been placed at the C terminus, a Tag:MYC tag at the N terminus. Expression of this construct is driven by UASt sequences.
When bi::org-1biN+org-1T+org-1C.Scer\UAS.T:Ivir\HA1,T:Hsap\MYC is driven by Scer\GAL4dpp.blk1 normal or slightly enlarged eyes are seen. When bi::org-1biN+org-1T+org-1C.Scer\UAS.T:Ivir\HA1,T:Hsap\MYC is driven by Scer\GAL4GMR.PF ommatidial development is impaired, and a dense growth of differently shaped microchaetae is seen in the eye field.