Heat induction of Msex\brZ4.hs at 37[o]C for 60 minutes results in the death of the Drosophila animal prior to the pharate adult stage. When Msex\brZ4.hs is induced at 33[o]C for 60 minutes viability is close to 100%.
Msex\brZ4.hs has lethal | pupal stage phenotype, suppressible by brrbp-1
Msex\brZ4.hs has leg phenotype, suppressible by brrbp-1
When one copy of the Msex\brZ4.hs transgene is expressed in brrbp-1 mutant flies, only 1.5% of brrbp-1 mutants are 'rescued' to eclosion. Flies carrying two doses of Msex\brZ4.hs experience approximately 8% rescue to eclosion. Mutant brrbp-1 flies rescued with Msex\brZ4.hs typically die within 1-2 days of eclosion. These animals have a protracted proboscis and are most likely unable to feed. The malformed leg phenotype found in brrbp-1 animals that develop to late pupal stages is rescued in approximately 66% of cases by the expression of two Msex\brZ4.hs transgenes.
