FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Cdc42V12.C40.UAS.Tag:MYC
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General Information
Symbol
Dmel\Cdc42V12.C40.UAS.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0184362
Feature type
allele
Associated gene
Dmel\Cdc42
Associated Insertion(s)
Carried in Construct
P{UAS-cdc42.V12C40.myc}
Also Known As
UAS-myc::Dcdc42V12C40
Key Links
Transgenic product class
missense_variant
(Kim et al., 2002)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Kim et al., 2002)
Mutagen
in vitro construct
(Kim et al., 2003, Kim et al., 2002)
Progenitor genotype
Carried in construct
P{UAS-cdc42.V12C40.myc}
Cytology
Description

UASt regulatory sequences drive expression of a mutated form of Cdc42 which carries the V12 constitutive activation mutation and in addition, a Y40C mutation in the effector loop. The protein is tagged with two copies of Tag:MYC.

(Kim et al., 2002)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Cdc42
Tagged with
Tag:MYC
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      In Cdc42V12.C40.Scer\UAS.T:Hsap\MYC; Scer\GAL4elav.PLu embryos, segmental nerve (SN)a and SNb motor neuron axons (abdominal posterior fascicle) and intersegmental nerve (ISN) motor neuron axons (anterior fascicle) all exhibit normal guidance.

      (Kim et al., 2003)

      Expression of Cdc42V12.C40.Scer\UAS.T:Hsap\MYC in SNb neurons under the control of Scer\GAL4elav-C155 results in a normal pathfinding pattern in the majority of cases, or stalling at or before muscle 6.

      SNd, SNa, and SNc axons expressing Scer\GAL4elav-C155-driven Cdc42V12.C40.Scer\UAS.T:Hsap\MYC display wild-type pathfinding patterns in 100% of cases.

      Expression of Cdc42V12.C40.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4elav.PLu increases LBD filopodial activity, with extension increased by 32% and retraction by 43%, respectively.

      SNb growth cones expressing Cdc42V12.C40.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4elav.PLurevert to wild-type pathfinding, but show no significant change in filopodial activity from wild-type. The only exception is a 20% increase in the number of filopodia directed posteriorly.

      (Kim et al., 2002)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Kim

      (Kim et al., 2003)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Cdc42V12.C40.Scer\UAS.T:Hsap\MYC
      Cdc42V12.C40.UAS.Tag:MYC
      Name Synonyms
      Secondary FlyBase IDs
        References (2)