FlyBase Allele Report: Dmel\upSET[e00365]

General Information

Symbol

Dmel\upSET^e00365

Species

D. melanogaster

Name

FlyBase ID

FBal0184684

Feature type

allele

Associated gene

Dmel\upSET

Associated Insertion(s)

PBac{RB}upSET^e00365

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

piggyBac activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Rincon-Arano et al., 2012)

Mutagen

piggyBac activity

(Gene Disruption Project members and Exelixis, 2005)

Progenitor genotype

Associated Insertion(s)

PBac{RB}upSET^e00365

Cytology

Description

Insertion in the second intron.

(Rincon-Arano et al., 2012)

Allele components

Component

Use(s)

Inserted element

PBac{RB}

gene trap

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:14,005,035..14,005,035 [+]

(Rincon-Arano et al., 2012, Gene Disruption Project members and Exelixis, 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile | recessive

(Rincon-Arano et al., 2012)

viable

(Rincon-Arano et al., 2012)

Phenotype Manifest In

egg chamber

(Rincon-Arano et al., 2012)

nurse cell

(Rincon-Arano et al., 2012)

Detailed Description

Statement

Reference

98% of homozygous females do not lay eggs.

80% of egg chambers do not progress beyond stage 8-9 in homozygous females. 13% of these egg chambers show anterior-posterior axis asymmetry, 25% have reduced numbers of nurse cells and 16% show egg chamber fusions. 80% of egg chambers that progress beyond stage 9 have abnormally large nucleoli.

(Rincon-Arano et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

upSET^e00365 has female sterile | recessive phenotype, suppressible | partially by Mmus\Mll5^UASp.cRa/Scer\GAL4^Act.PU

(Rincon-Arano et al., 2012)

Enhancer of

Statement

Reference

CG9007[+]/upSET^e00365 is an enhancer of homeotic | dominant | male phenotype of Pc¹

(Rincon-Arano et al., 2012)

CG9007[+]/upSET^e00365 is an enhancer of homeotic | dominant | male phenotype of Pc³

(Rincon-Arano et al., 2012)

Suppressor of

Statement

Reference

CG9007[+]/upSET^e00365 is a suppressor of homeotic | dominant phenotype of brm[+]/brm², trx^E2

(Rincon-Arano et al., 2012)

Phenotype Manifest In

Enhancer of

Statement

Reference

CG9007[+]/upSET^e00365 is an enhancer of sex comb | ectopic | male phenotype of Pc¹

(Rincon-Arano et al., 2012)

CG9007[+]/upSET^e00365 is an enhancer of sex comb | ectopic | male phenotype of Pc³

(Rincon-Arano et al., 2012)

Suppressor of

Statement

Reference

CG9007[+]/upSET^e00365 is a suppressor of metathoracic leg phenotype of brm[+]/brm², trx^E2

(Rincon-Arano et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The penetrance of the ectopic sex comb phenotype seen on the second and/or third legs of Pc³/+ flies (40%) is enhanced if they are also heterozygous for upSET^e00365 (89%).

The penetrance of the ectopic sex comb phenotype seen on the second and/or third legs of Pc¹/+ flies (33%) is enhanced if they are also heterozygous for upSET^e00365.

56% of brm² trx^E2 double heterozygotes have an apical bristle on the third leg (usually found on the second leg). Heterozygosity for upSET^e00365 reduces the penetrance of this phenotype to 9%.

(Rincon-Arano et al., 2012)

Xenogenetic Interactions

Statement

Reference

Expression of Mmus\Mll5^{Scer\UAS.P\T.cRa} under the control of Scer\GAL4^Act.PU partially rescues the sterility of upSET^e00365 homozygous females.

(Rincon-Arano et al., 2012)

Complementation and Rescue Data

Rescued by

upSET^e00365 is rescued by upSET^{+tCH322-187J08}

(Rincon-Arano et al., 2012)

Comments

Two copies of upSET^{+tCH322-187J08} rescue the sterility of upSET^e00365 homozygous females.

(Rincon-Arano et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG9007^e00365

upSET^e00365

(Rincon-Arano et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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