FlyBase Allele Report: Dmel\ArfGAP3[e01250]

General Information

Symbol

Dmel\ArfGAP3^e01250

Species

D. melanogaster

Name

FlyBase ID

FBal0185122

Feature type

allele

Associated gene

Dmel\ArfGAP3

Associated Insertion(s)

PBac{RB}ArfGAP3^e01250

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

piggyBac activity

Nature of the Allele

Allele class

Mutagen

piggyBac activity

(Gene Disruption Project members and Exelixis, 2005)

Progenitor genotype

Associated Insertion(s)

PBac{RB}ArfGAP3^e01250

Cytology

Description

Allele components

Component

Use(s)

Inserted element

PBac{RB}

gene trap

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:22,717,451..22,717,451 [+]

(Johnson et al., 2011, Gene Disruption Project members and Exelixis, 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

ommatidium

(Johnson et al., 2011)

retina

(Johnson et al., 2011)

Detailed Description

Statement

Reference

Flies homozygous for ArfGAP3^e01250 that survive to 27 h after puparium formation (APF) display mild patterning defects in their retina.

(Johnson et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

ArfGAP3^e01250/CG6838[+] is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Johnson et al., 2011)

ArfGAP3^e01250/CG6838[+] is an enhancer of retina phenotype of Arf6^GD13822, Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF

(Johnson et al., 2011)

ArfGAP3^e01250/CG6838[+] is an enhancer of ommatidium phenotype of Arf6^GD13822, Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF

(Johnson et al., 2011)

ArfGAP3^e01250/CG6838[+] is an enhancer of primary pigment cell phenotype of Arf6^GD13822, Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF

(Johnson et al., 2011)

ArfGAP3^e01250/CG6838[+] is an enhancer of secondary pigment cell phenotype of Arf6^GD13822, Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF

(Johnson et al., 2011)

ArfGAP3^e01250/CG6838[+] is an enhancer of tertiary pigment cell phenotype of Arf6^GD13822, Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF

(Johnson et al., 2011)

ArfGAP3^e01250/CG6838[+] is an enhancer of cone cell phenotype of Arf6^GD13822, Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF

(Johnson et al., 2011)

ArfGAP3^e01250/CG6838[+] is an enhancer of interommatidial precursor cell phenotype of Arf6^GD13822, Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF

(Johnson et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The eye mis-patterning phenotype resulting from the co-expression of Arf51F^GD13822 with Dcr-2^Scer\UAS.cDa under the control of Scer\GAL4^GMR.PF is significantly enhanced in ArfGAP3^e01250 heterozygotes. The number of correctly patterned secondary and tertiary cell is reduced. The total number of interommatidial precursor cells (IPCs) does not differ markedly from the wild-type number of 12, indicating that the enhancement is due to specifically to increased disorder in IPC patterning. Additionally, errors in the placement of three bristle groups about each ommatidium and errors in primary pigment cell and cone cell patterning and ommatidial rotation are modestly enhanced.

The ommatidial patterning errors resulting from the expression of cindr^{dsRNA.PC.PD.Scer\UAS} via Scer\GAL4^GMR.PF are strongly increased in ArfGAP3^e01250 heterozygotes.

(Johnson et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

86009

w¹¹¹⁸; PBac{RB}ArfGAP3^e01250

Notes on Origin

Discoverer

Separable from: PBac{RB}e01250.

(Gene Disruption Project members and Exelixis, 2005)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ArfGAP3^e01250

CG6838^e01250

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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