FlyBase Allele Report: Dmel\Vps13[c03628]

General Information

Symbol

Dmel\Vps13^c03628

Species

D. melanogaster

Name

FlyBase ID

FBal0186667

Feature type

allele

Associated gene

Dmel\Vps13

Associated Insertion(s)

PBac{PB}Vps13^c03628

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

piggyBac activity

Nature of the Allele

Allele class

Mutagen

piggyBac activity

(Gene Disruption Project members and Exelixis, 2005)

Progenitor genotype

Associated Insertion(s)

PBac{PB}Vps13^c03628

Cytology

Description

Allele components

Component

Use(s)

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:7,573,841..7,573,841 [-]

(Yeshaw et al., 2019, Gene Disruption Project members and Exelixis, 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of choreaacanthocytosis

CEA

(Yeshaw et al., 2019, Vonk et al., 2017)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of choreaacanthocytosis

is ameliorated by Hsap\VPS13A^UAS.1A

(Yeshaw et al., 2019, Vonk et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage | progressive | recessive

(Vonk et al., 2017)

abnormal neuroanatomy | adult stage | recessive

(Vonk et al., 2017)

decreased fecundity | female

(Faber et al., 2020)

decreased fecundity | female (with Vps13^null)

(Faber et al., 2020)

decreased size | female

(Faber et al., 2020)

partially lethal | heat sensitive (with Df(2R)Exel6052)

(Vonk et al., 2017)

partially lethal | heat sensitive (with Df(2R)Exel6053)

(Vonk et al., 2017)

partially lethal | recessive | heat sensitive

(Vonk et al., 2017)

short lived

(Faber et al., 2020)

short lived | recessive

(Vonk et al., 2017)

viable

(Vonk et al., 2017)

Phenotype Manifest In

adult brain

(Vonk et al., 2017)

adult central complex

(Vonk et al., 2017)

egg chamber

(Faber et al., 2020)

egg chamber (with Vps13^null)

(Faber et al., 2020)

nucleus | ectopic | female

(Faber et al., 2020)

nucleus | ectopic | female (with Vps13^null)

(Faber et al., 2020)

nurse cell

(Faber et al., 2020)

nurse cell (with Vps13^null)

(Faber et al., 2020)

ovary

(Faber et al., 2020)

Detailed Description

Statement

Reference

Vps13^c03628 homozygotes are viable at 22[o]C but partially lethal at higher temperatures, with significantly fewer animals eclosing at 29[o]C] than at 25[o]C; at 25[o]C, the surviving adults present a significant decrease in lifespan, with the majority of individuals dying between days 16 and 20 post eclosion, as compared to wild-type controls; Vps13^c03628 homozygous adults present a significant decrease in climbing capacity at day 17 (but not at day 4) post eclosion, as compared to wild-type age-matched controls, and present abundant vacuoli in the brain, including in the central complex, at day 20 post eclosion, which is absent in wild-type age-matched controls; Vps13^c03628 heterozygotes do not show any obvious phenotype, as compared to controls.

Vps13^c03628/Df(2R)Exel6053 and Vps13^c03628/Df(2R)Exel6052 transheterozygotes reared at 29[o]C are partially lethal, showing a significant decrease in the eclosion rate as compared to controls.

(Vonk et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Vps13^c03628 has partially lethal | recessive | heat sensitive phenotype, suppressible | partially by Scer\GAL4^Act5C.PI/Hsap\VPS13A^UAS.1A

(Vonk et al., 2017)

Vps13^c03628 has short lived phenotype, suppressible | partially by Scer\GAL4^Act5C.PI/Hsap\VPS13A^UAS.1A

(Vonk et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Vps13^c03628 has egg chamber phenotype, suppressible | partially by Scer\GAL4^Act5C.PI/Hsap\VPS13A^UAS.1A

(Faber et al., 2020)

Vps13^c03628 has nurse cell phenotype, suppressible | partially by Scer\GAL4^Act5C.PI/Hsap\VPS13A^UAS.1A

(Faber et al., 2020)

Vps13^c03628 has nucleus | ectopic | female phenotype, suppressible | partially by Scer\GAL4^Act5C.PI/Hsap\VPS13A^UAS.1A

(Faber et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The decreased lifespan and the partial lethality observed in Vps13^c03628 homozygotes reared at 25[o]C is partially suppressed by the expression of Hsap\VPS13A^Scer\UAS.1A under the control of Scer\GAL4^Act5C.PI.

(Vonk et al., 2017)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG2093^c03628

Vps13^PB

(Faber et al., 2020)

Vps13^c03628

(Faber et al., 2020, Vonk et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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