FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\eagG297E
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General Information
Symbol
Dmel\eagG297E
Species
D. melanogaster
Name
FlyBase ID
FBal0189953
Feature type
allele
Associated gene
Dmel\eag
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

gain of function allele

Mutagen
Nature of the Allele
Allele class

gain of function allele

(Cardnell et al., 2006)
Mutagen
ethyl methanesulfonate
(Cardnell et al., 2006)
Progenitor genotype
Cytology
Description

Amino acid replacement: G297E.

(Cardnell et al., 2006, Cardnell et al., 2006)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
X:14,995,979..14,995,979 [+]
Nucleotide change:

G14995979A

Amino acid change:

G297E | eag-PA; G297E | eag-PB; G280E | eag-PC; G295E | eag-PD; G278E | eag-PE; G287E | eag-PF; G280E | eag-PG

Reported amino acid change:

G297E

Comment:

Site of nucleic acid difference inferred by FlyBase based on reported amino acid change.

(Cardnell et al., 2006, Cardnell et al., 2006)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      gain of function

      (Cardnell et al., 2006)

      eagG297E flies do not show temperature-sensitive paralysis, the flies are well coordinated and their flight appears normal.

      The amplitude and duration of the excitatory junctional potential (ejp) elicited in the muscle at the neuromuscular junction in eagG297E larvae at 0.15 mM CaCl2 is not significantly affected compared to wild type. The onset rate of long-term facilitation is also unaffected in the mutants.

      (Cardnell et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      eagG297E is a suppressor of abnormal neurophysiology phenotype of Sh14

      (Cardnell et al., 2006)

      eagG297E is a suppressor of increased rate of movement | dominant phenotype of Sh14

      (Cardnell et al., 2006)

      eagG297E/eag[+] is a suppressor of abnormal neurophysiology phenotype of Sh14

      (Cardnell et al., 2006)

      eagG297E/eagG297E is a suppressor of abnormal neurophysiology phenotype of Sh14

      (Cardnell et al., 2006)
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      eagG297E is a suppressor of leg phenotype of Sh14

      (Cardnell et al., 2006)

      eagG297E/eag[+] is a suppressor of embryonic/larval neuromuscular junction phenotype of Sh14

      (Cardnell et al., 2006)

      eagG297E/eagG297E is a suppressor of embryonic/larval neuromuscular junction phenotype of Sh14

      (Cardnell et al., 2006)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The ether-induced leg shaking of Sh14 mutants is suppressed by eagG297E.

      (Cardnell et al., 2006)

      eagG297E suppresses the mutant excitatory junctional potential (ejp) phenotype that is seen at low Ca2+ levels (0.1mM) in Sh14 larvae in a dosage dependent manner; eagG297E/+ results in a moderate decrease in the ejp amplitude, but not ejp duration, while eagG297E/eagG297E significantly decreases both ejp amplitude and duration. This suppression requires extracellular Mg2+.

      The average amplitude of the excitatory junctional current (ejc) at the neuromuscular junction is significantly smaller in eagG297E Sh14 animals than in eag+ Sh14 animals. The average amplitude of the spontaneous miniature excitatory junctional potential (mejp) is almost identical in eagG297E Sh14 and eag+ Sh14 animals.

      (Cardnell et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Induced on: Sh14.

      (Cardnell et al., 2006)

      Selected as: A suppressor of the leg-shaking phenotype seen under ether anesthesia in Sh14/+ flies.

      (Cardnell et al., 2006)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      Sup146
      (Cardnell et al., 2006)
      Sup39
      (Cardnell et al., 2006)
      eag146
      (Cardnell et al., 2006)
      eag39
      (Cardnell et al., 2006)
      eagG297E
      Name Synonyms
      Secondary FlyBase IDs
        References (2)