The genomic sequence of Ctr1B, including upstream metal response elements and introns and the complete coding sequence, is fused upstream of a EGFP tag. All four metal response elements have been mutated.
Ctr1Bm1-m2-m3-m4.EGFP fails to rescue Ctr1B3-4
Ctr1Bm1-m2-m3-m4.T:Avic\GFP-EGFP does not rescue the low copper lethality phenotype of Ctr1B3-4.