Low expression lines (P{βTub85D-SigD}R1 and P{βTub85D-SigD}R1) have superficially normal testes, whereas high expression lines have severe phenotypes.

Males with the R1 insertion (P{βTub85D-SigD}R1) have defects in spermatid cyst elongation and polarity: early elongating cysts have scattered nuclei and later stage cysts are much shorter than wild type and appear bi-polar with nuclei distributed between the two ends and sperm tails growing toward the middle of the cyst. Axonemes grow from opposite directions.

Individual elongating P{βTub85D-SigD}R1 spermatids are not polarized correctly.

Plasma membranes separating individual spermatids are largely absent in P{βTub85D-SigD}R1 cysts.

Testes expressing Styp\sopB^{ΔN29.βTub85D} show accumulation of ovoid spermatid cysts, and lack elongating or individualising spermatids. Microtubule organization is disorganised. There are few axonemes, and these are often oriented in different directions. Ensheathment of the axoneme by the axial membrane is often compromised and the sheath is typically dissociated from the mitochondrial derivative. Basal bodies are typically mislocalised, often lying tangential to the nuclear envelope. There is a delay in axoneme assembly. In contrast to wildtype, the basal body is not embedded in the nuclear envelope.

Expression of the Styp\sopB^{ΔN29.βTub85D} transgene causes a weak cytokinesis defect, with 1.1-1.9% of spermatids containing multiple nuclei, although the majority contain two nuclei rather than four.

Styp\sopB^{ΔN29.βTub85D} has spermatid cyst phenotype, suppressible | partially by sktl^{βTub85D.EYFP}

Styp\sopB^{ΔN29.βTub85D} has spermatid cyst phenotype, suppressible by sktl^{βTub85D.EYFP}

Styp\sopB^{ΔN29.βTub85D} has elongation stage spermatid phenotype, suppressible by sktl^{βTub85D.EYFP}

Styp\sopB^{ΔN29.βTub85D} has microtubule phenotype, suppressible | partially by sktl^{βTub85D.EYFP}

Styp\sopB^{ΔN29.βTub85D} has axoneme phenotype, suppressible by sktl^{βTub85D.EYFP}

Styp\sopB^{ΔN29.βTub85D} has ciliary basal body phenotype, suppressible | partially by sktl^{βTub85D.EYFP}