FlyBase Allele Report: Dmel\mir-7[UAS.cLa]

General Information

Symbol

Dmel\mir-7^UAS.cLa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Lai

FlyBase ID

FBal0190939

Feature type

allele

Associated gene

Dmel\mir-7

Associated Insertion(s)

Carried in Construct

P{UAS-mir-7.L}

Also Known As

UAS-miR-7

Key Links

Transgenic product class

wild_type

(Lai et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Lai et al., 2005)

Mutagen

in vitro construct

(Bejarano et al., 2012, Aboobaker et al., 2005, Lai et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-mir-7.L}

Cytology

Description

UAS regulatory sequences drive expression of mir-7.

(Lai et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

mir-7

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^Bx-MS1096

(Bejarano et al., 2012)

visible, with Scer\GAL4^dpp.blk1

(Lai et al., 2005)

visible, with Scer\GAL4^sd-SG29.1

(Bejarano et al., 2012)

Phenotype Manifest In

first posterior wing cell, with Scer\GAL4^dpp.blk1

(Lai et al., 2005)

wing, with Scer\GAL4^Bx-MS1096

(Bejarano et al., 2012)

wing, with Scer\GAL4^dpp.blk1

(Lai et al., 2005)

wing, with Scer\GAL4^ptc-559.1

(Chou et al., 2007, Aboobaker et al., 2005)

wing, with Scer\GAL4^sd-SG29.1

(Bejarano et al., 2012)

wing cell, with Scer\GAL4^ptc-559.1

(Chou et al., 2007)

wing margin, with Scer\GAL4^sd-SG29.1

(Bejarano et al., 2012)

wing vein, with Scer\GAL4^sd-SG29.1

(Bejarano et al., 2012)

Detailed Description

Statement

Reference

Expression of mir-7^Scer\UAS.cLa under the control of Scer\GAL4^Bx-MS1096 results in wing defects such as curving or blistering.

Expression of mir-7^Scer\UAS.cLa under the control of Scer\GAL4^sd-SG29.1 results in loss of wing margin, a reduction in wing size and thicker veins than normal.

(Bejarano et al., 2012)

Wings expressing mir-7^Scer\UAS.cLa under the control of Scer\GAL4^ptc-559.1 exhibit wing defects, including a distal wing notch and loss of the proximal L3/L4 domain.

(Chou et al., 2007)

mir-7^Scer\UAS.cLa mutants (under the activation of Scer\GAL4^ptc-559.1)exhibit wing notching and bristle tufting.

(Aboobaker et al., 2005)

Expression of mir-7^Scer\UAS.cLa under the control of Scer\GAL4^dpp.blk1 results in a notched wing and a reduced L3-L4 intervein region. More generalised expression of mir-7^Scer\UAS.cLa through Scer\GAL4^Bx-MS1096 induces strong thickening of wing veins.

(Lai et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Scer\GAL4^ptc-559.1, mir-7^UAS.cLa has wing phenotype, non-suppressible by FHV\B2^UAS.HM, Scer\GAL4^ptc-559.1

(Chou et al., 2007)