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General Information
Symbol
Dmel\PargUAS.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0191060
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS::Parg-EGFP
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of full-length Parg, which is tagged with EGFP.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Flies that express PargScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 are lethal; stage of lethality is dependent on insertion site and dosage of the transgene and driver. Cells expressing PargScer\UAS.T:Avic\GFP-EGFP, under the control of Scer\GAL4da.G32, show fragmented nucleoli. Third instar larvae expressing PargScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 fail to recover from a 40-min heat shock to the same extent as wild-type larvae (60% of mutants survive vs 99% of controls).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The lethality that results from expression of PargScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4da.G32 is rescued when ParpI.Scer\UAS.T:Disc\RFP is coexpressed.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of PargScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL469B rescues the lethality of Parg27.1 animals.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
PargScer\UAS.T:Avic\GFP-EGFP
PargUAS.EGFP
Name Synonyms
Secondary FlyBase IDs
    References (3)