FlyBase Allele Report: Dmel\brk[UAS.cMa]

General Information

Symbol

Dmel\brk^UAS.cMa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Moser

FlyBase ID

FBal0191282

Feature type

allele

Associated gene

Dmel\brk

Associated Insertion(s)

Carried in Construct

P{UAS(-FRT)brk.B459}

Key Links

Transgenic product class

wild_type

(Moser and Campbell, 2005)

Mutagen

FLPase

Nature of the Allele

Transgenic product class

wild_type

(Moser and Campbell, 2005)

Mutagen

FLPase

(Moser and Campbell, 2005)

Progenitor genotype

brk^FRT.UAS.B459

(Moser and Campbell, 2005)

Carried in construct

P{UAS(-FRT)brk.B459}

Cytology

Description

The FRT cassette has been excised, juxtaposing the brk coding and UAS regulatory sequences.

(Moser and Campbell, 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

brk

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

posterior crossvein | heat sensitive, with Scer\GAL4^en-e16E

(Moser and Campbell, 2005)

wing | posterior, with Scer\GAL4^en-e16E

(Moser and Campbell, 2005)

wing | posterior | heat sensitive, with Scer\GAL4^en-e16E

(Moser and Campbell, 2005)

wing disc, with Scer\GAL4^en-e16E

(Moser and Campbell, 2005)

wing margin | heat sensitive, with Scer\GAL4^en-e16E

(Moser and Campbell, 2005)

wing vein L4, with Scer\GAL4^en-e16E

(Moser and Campbell, 2005)

wing vein L4 | heat sensitive, with Scer\GAL4^en-e16E

(Moser and Campbell, 2005)

wing vein L5, with Scer\GAL4^en-e16E

(Moser and Campbell, 2005)

Detailed Description

Statement

Reference

At 20^oC, expression of brk^Scer\UAS.cMa in the posterior of the wing disc, driven by Scer\GAL4^en-e16E, produces a mild phenotype in adult wings. The posterior of the wing is slightly reduced compared to wild type and wing veins 4 and 5 are incomplete at the distal end. When flies of the same genotype are raised at 25^oC, the wing phenotype is more severe; the posterior is more reduced in size, the wing margin is notched distally, vein 4 and the posterior crossvein are lost, and vein 5 is incomplete distally. Examination of wing discs of this genotype shows that disc shape is altered relative to wild type; at 25^oC, the discs have a deep invagination between brk^Scer\UAS.cMa-expressing cells in the posterior and non brk^Scer\UAS.cMa-expressing cells in the anterior. At 20^oC, this effect is reduced, but a small invagination is still observed.

(Moser and Campbell, 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Scer\GAL4^en-e16E, brk^UAS.cMa has wing vein L4 phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^en-e16E

(Moser and Campbell, 2005)

Scer\GAL4^en-e16E, brk^UAS.cMa has posterior crossvein | heat sensitive phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^en-e16E

(Moser and Campbell, 2005)

Scer\GAL4^en-e16E, brk^UAS.cMa has wing margin | heat sensitive phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^en-e16E

(Moser and Campbell, 2005)

Scer\GAL4^en-e16E, brk^UAS.cMa has wing vein L4 | heat sensitive phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^en-e16E

(Moser and Campbell, 2005)

Scer\GAL4^en-e16E, brk^UAS.cMa has wing vein L5 phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^en-e16E

(Moser and Campbell, 2005)

Scer\GAL4^en-e16E, brk^UAS.cMa has wing | posterior | heat sensitive phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^en-e16E

(Moser and Campbell, 2005)

Scer\GAL4^en-e16E, brk^UAS.cMa has wing | posterior phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^en-e16E

(Moser and Campbell, 2005)

Scer\GAL4^en-e16E, brk^UAS.cMa has wing phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^en-e16E

(Moser and Campbell, 2005)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, brk^UAS.cMa has wing phenotype

(Moser and Campbell, 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The wing defects seen in flies expressing brk^Scer\UAS.cMa are not due to excessive cell death as preventing cell death by overexpression of BacA\p35^Scer\UAS.cHa does not suppress the phenotype. Flies co-expressing brk^Scer\UAS.cMa and BacA\p35^Scer\UAS.cHa, both under the control of Scer\GAL4^en-e16E, at 20^oC show the same phenotype as flies that express only brk^Scer\UAS.cMa. When brk^Scer\UAS.cMa and BacA\p35^Scer\UAS.cHa are co-expressed at 25⁰C, again driven by Scer\GAL4^en-e16E, there is a slight blistering of the adult wing at the posterior, indicating that extra wing tissue is present when cell death is prevented. However, the wing vein defects and notching still occur.

(Moser and Campbell, 2005)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

brk^Scer\UAS.cMa

brk^UAS.cMa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Moser

(Moser and Campbell, 2005)

Secondary FlyBase IDs

References (1)

Report Sections

Open Close