FlyBase Allele Report: Dmel\Cyt-c-d[Z21091]

General Information

Symbol

Dmel\Cyt-c-d^Z21091

Species

D. melanogaster

Name

FlyBase ID

FBal0191544

Feature type

allele

Associated gene

Dmel\Cyt-c-d

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Arama et al., 2006)

Progenitor genotype

Cytology

Description

Nucleotide substitution: G?A.

(Arama et al., 2006)

The premature stop codon results in a truncated protein that is almost half the size of the wild-type protein.

(Arama et al., 2006)

Amino acid replacement: W62term.

(Arama et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:16,719,320..16,719,320 [+]

Nucleotide change:

G16719320A

Amino acid change:

W62term | Cyt-c-d-PA; W62term | Cyt-c-d-PB

Reported amino acid change:

W62term

Comment:

TGG to TGA

(Arama et al., 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

male sterile

(Arama et al., 2006)

visible

(Mendes et al., 2006)

Phenotype Manifest In

posterior scutellar bristle | increased number

(Mendes et al., 2006)

secondary pigment cell

(Mendes et al., 2006)

secondary pigment cell (with Df(2L)H20)

(Mendes et al., 2006)

spermatid

(Arama et al., 2006)

tertiary pigment cell

(Mendes et al., 2006)

tertiary pigment cell (with Df(2L)H20)

(Mendes et al., 2006)

Detailed Description

Statement

Reference

Cyt-c-d^Z21091 homozygotes do not exhibit a significant proportion of hyperplastic testes, as compared to controls.

(Napoletano et al., 2017)

Cyt-c-d^Z21091 mutants show defects in spermatid individualization.

(Arama et al., 2006)

At 42 hours after puparium formation (APF), homozygous and Cyt-c-d^Z21091/Df(2L)H20 retinas have extra interommatidial cells in the secondary or tertiary position compared to wild type. At 22 hours APF the number of extra interommatidial cells is significantly higher than at 42 hours APF. Despite the presence of extra interommatidial cells in the retina, the process of cell sorting and interommatidial cell maturation occurs similarly to wild type in the mutant animals (20-27 hours APF).

The cell death of perimeter ommatidia at the edge of the eye, that normally occurs between 36 and 44 hours APF, is delayed in homozygous flies. At 40 hours APF, the mutant retinal edges have more clusters of malformed ommatidia in a thick later of interommatidial cells (while in wild type, many perimeter ommatidial clusters have been eliminated by this stage). By 54 hours APF, elimination of perimeter ommatidial clusters is complete in both mutant and wild-type animals.

Homozygous adult eyes have the normal number and arrangement of photoreceptor cells.

Homozygous flies have extra posterior scutellar bristles compared to wild type.

(Mendes et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Cyt-c-d^Z21091 has male sterile phenotype, suppressible by Cyt-c-p^k13905

(Arama et al., 2006)

Suppressor of

Statement

Reference

Cyt-c-d^Z21091 is a suppressor of lethal phenotype of Cyt-c-p^k13905

(Arama et al., 2006)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Cyt-c-d^Z21091 complements the lethality of Cyt-c-p^k13905, and Cyt-c-p^k13905 complements the sterility of Cyt-c-d^Z21091.

(Arama et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Cyt-c-d^bln1

(Arama et al., 2006)

Rescued by

Cyt-c-d^Z21091 is rescued by Cyt-c-d^GMR.PM

(Mendes et al., 2006)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

C. Zuker

(Arama et al., 2006)

Selected as: a male sterile mutant defective in spermatid individualization.

(Arama et al., 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Cyt-c-d^Z21091

cyt c-d^Z2-1091

(Mendes et al., 2006)

cyt-c-d^Z2-1091

(Arama et al., 2006)

cyt-c-d^Z21091

(Napoletano et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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