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General Information
Symbol
Avic\GFPEGFP.E(spl)m8-HLH
Species
A. victoria
Name
FlyBase ID
FBal0191746
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
E(spl)m8-EGFP, m8-GFP
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

An E(spl)m8-HLH enhancer and a minimal Hsp70 promoter drive expression of EGFP tagged with a nuclear localization signal (Tag:NLS(tra)). gypsy\su(Hw)BR insulator sequences flank the reporter cassette. The E(spl)m8-HLH enhancer is a 1.1kb genomic EcoRI-XhoI fragment that corresponds to -1174 to -72 of the E(spl)m8-HLH upstream region.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
distribution deduced from reporter or direct label
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Reporter expression is observed in the NB3-3A neuroblast at embryonic stage 13.

At embryonic stages 10 and 11 Avic\GFPEGFP.E(spl)m8-HLH is very weakly expressed in neuroblasts NB5-6 of the embryonic meso-and metathorax. The expression increases towards stage 15.

 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Avic\GFPE(spl).H-Stinger
Avic\GFPE(spl)m8-HLH.H-Stinger
Avic\GFPEGFP.E(spl)m8-HLH
Name Synonyms
Secondary FlyBase IDs
    References (6)