FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Sp7RNAi.UAS
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General Information
Symbol
Dmel\Sp7RNAi.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0193154
Feature type
allele
Associated gene
Dmel\Sp7
Associated Insertion(s)
Carried in Construct
P{UAS-Sp7.RNAi}
Key Links
Transgenic product class
RNAi_reagent
(Castillejo-Lopez and Häcker, 2005)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Castillejo-Lopez and Häcker, 2005)
Mutagen
in vitro construct
(Castillejo-Lopez and Häcker, 2005)
Progenitor genotype
Carried in construct
P{UAS-Sp7.RNAi}
Cytology
Description

UASt regulatory sequences drive expression of a 729bp genomic fragment of Sp7 (which includes the last 3 introns) and a 425bp cDNA sequence of Sp7 arranged in opposite orientation. The construct is expected to produce a dsRNA composed of a 425bp hairpin linked by a 148 nucleotide loop.

(Castillejo-Lopez and Häcker, 2005)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Sp7
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Sp7dsRNA.Scer\UAS in crystal cells under the control of Scer\GAL4lz-gal4 results in reduced hemolymph clot melanization.

      (Bidla et al., 2007)

      Flies expressing Sp7dsRNA.Scer\UAS under the control of Scer\GAL4tub show a severe reduction in the amount of melanisation produced at a wound site (flies were pricked with a needle dipped in a concentrated mixture of E.coli and M.luteus) compared to wild-type flies.

      Spontaneous melanisation of the hemolymph (assayed by dissecting larvae and exposing them to air) is much lower in flies expressing Sp7dsRNA.Scer\UAS under the control of Scer\GAL4unspecified larvae than in wild type.

      (Castillejo-Lopez and Häcker, 2005)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Used to study the phenotypic consequences of dsRNA interference (RNAi) of the Sp7 gene.

      (Castillejo-Lopez and Häcker, 2005)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Sp7RNAi.UAS
      Sp7dsRNA.Scer\UAS
      Sp7dsRNA.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (3)