FlyBase Gene Report: Dmel\Sp7

General Information

Symbol

Dmel\Sp7

Species

D. melanogaster

Name

Serine protease 7

Annotation Symbol

CG3066

Feature Type

protein_coding_gene

FlyBase ID

FBgn0037515

Gene Model Status

Current

Stock Availability

11 publicly available

Gene Summary

Serine protease 7 (Sp7) encodes a hemolymphatic serine protease involved in the activation of the melanization cascade. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

MP2, PAE1, Melanization Protease 2

Key Links

Genomic Location

Cytogenetic map

84D14-84E1

Sequence location

3R:7,759,786..7,763,947 [+]

Recombination map

3-48

RefSeq locus

NT_033777 REGION:7759786..7763947

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (7 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables serine-type endopeptidase activity

inferred from electronic annotation with InterPro:IPR001254, InterPro:IPR018114

(InterPro Project Members, 2004-)

inferred from sequence model

(Cao and Jiang, 2018, Kelleher and Pennington, 2009, Ross et al., 2003)

inferred from biological aspect of ancestor with PANTHER:PTN001207268

(GO Reference Genome Project, 2011-)

Biological Process (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in defense response to Gram-positive bacterium

inferred from mutant phenotype

(Dudzic et al., 2019)

involved_in melanization defense response

inferred from mutant phenotype

(Bidla et al., 2007, Tang et al., 2006)

involved_in positive regulation of melanization defense response

inferred from genetic interaction with FLYBASE:PPO1; FB:FBgn0283437

(Dudzic et al., 2019)

inferred from mutant phenotype

(Castillejo-Lopez and Häcker, 2005)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in proteolysis

inferred from biological aspect of ancestor with PANTHER:PTN001207268

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR001254, InterPro:IPR018114

(InterPro Project Members, 2004-)

inferred from sequence model

(Kelleher and Pennington, 2009, Ross et al., 2003)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in extracellular region

inferred from high throughput direct assay

(Karlsson et al., 2004)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in extracellular space

inferred from biological aspect of ancestor with PANTHER:PTN001207268

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

S1A SERINE PROTEASES - TRYPSIN-LIKE

Protein Family (UniProt)

Belongs to the peptidase S1 family. CLIP subfamily. (Q9V3Z2)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Serine protease 7 (Sp7) encodes a hemolymphatic serine protease involved in the activation of the melanization cascade. [Date last reviewed: 2019-03-14]

Gene Group (FlyBase)

S1A SERINE PROTEASES - TRYPSIN-LIKE -: Trypsin-like serine proteases of the S1A family are endopeptidases that cleave amide substrates following Arginine or Lysine at the P1 position. (Adapted from FBrf0239469).

Protein Function (UniProtKB)

Serine protease that, by cleaving and activating prophenoloxidase (PPO1) after immune challenge, plays an essential role in the melanization immune response to septic wounding (PubMed:16256951, PubMed:16322759, PubMed:16861233, PubMed:19071960, PubMed:24260243). May function in diverse Hayan-dependent PPO1-activating cascades that are negatively controlled by different serpin proteins; Spn27A in the hemolymph and Spn77BA in the trachea (PubMed:16322759, PubMed:18854145, PubMed:24260243). Important for the innate immune response to fungi (PubMed:16861233). Regulation of melanization and PPO1 activation appears to be largely independent of the Toll signaling pathway (PubMed:16861233).

(UniProt, Q9V3Z2)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Sp7 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9V3Z2)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.49

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 6.03

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Sp7-RA

FBtr0081740

NM_141477

1851

391

Sp7-RE

FBtr0339180

NM_169192

1418

391

Sp7-RF

FBtr0339181

NM_169193

1939

391

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Sp7-PA

FBpp0081237

43.1

391

6.59

FBpp0308321

43.1

391

6.59

FBpp0308322

43.1

391

6.59

Q9V3Z2

NP_731175

AAG22127

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

391 aa isoforms: Sp7-PA, Sp7-PE, Sp7-PF

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with Spn27A.

(UniProt, Q9V3Z2)

Domain

The clip domain consists of 35-55 residues which are 'knitted' together usually by 3 conserved disulfide bonds forming a clip-like compact structure.

(UniProt, Q9V3Z2)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9V3Z2

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Sp7 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Sp7_1
Sp7_2

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.63

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in extracellular region

inferred from high throughput direct assay

(Karlsson et al., 2004)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Sp7 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Sp7

View exonic expression by tissue for Dmel\Sp7

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40918/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0037515

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0037515

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0037515

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3066

Flygut - An atlas of the Drosophila adult midgut

FBgn0037515

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 4 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 15 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Sp7

Transgenic constructs containing regulatory region of Sp7

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 8 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Act.PU

Sp7^RNAi.UAS.WIZ

lethal, with Scer\GAL4^da.G32

Sp7^{Δ.UAS.Tag:SS(ea)}

lethal - all die before end of pupal stage, with Scer\GAL4^Act.PU

Sp7^{UASp.Tag:SS(ea)}

partially lethal, with Scer\GAL4^Act.PU

Sp7^RNAi.UAS

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Sp7^GD468

viable, with Scer\GAL4^Mef2.PR

Sp7^GD468

viable, with Scer\GAL4^pnr-MD237

Sp7^GD468

viable, with Scer\GAL4^unspecified

Sp7^RNAi.UAS.cMa

Other Phenotypes

Allele

abnormal immune response, with Scer\GAL4^da.G32

Sp7^RNAi.UAS.WIZ

abnormal immune response | adult stage

Sp7^KG02818

abnormal immune response | larval stage

Sp7^SK6

abnormal wound healing, with Scer\GAL4^Act.PU

Sp7^RNAi.UAS.WIZ

melanotic mass phenotype, with Scer\GAL4^da.G32

Sp7^{Δ.UAS.Tag:SS(ea)}

melanotic mass phenotype | adult stage, with Scer\GAL4^Act.PU

Sp7^{UASp.Tag:SS(ea)}

melanotic mass phenotype | larval stage, with Scer\GAL4^Act.PU

Sp7^{UASp.Tag:SS(ea)}

melanotic mass phenotype | pupal stage, with Scer\GAL4^Act.PU

Sp7^{UASp.Tag:SS(ea)}

short lived | conditional

Sp7^KG02818

some die during pupal stage, with Scer\GAL4^Act.PU

Sp7^{UASp.Tag:SS(ea)}

Phenotype manifest in

Allele

embryonic/larval hemolymph | third instar larval stage, with Scer\GAL4^lz-gal4

Sp7^RNAi.UAS

melanotic mass, with Scer\GAL4^da.G32

Sp7^{Δ.UAS.Tag:SS(ea)}

melanotic mass | adult stage, with Scer\GAL4^Act.PU

Sp7^{UASp.Tag:SS(ea)}

melanotic mass | larval stage, with Scer\GAL4^Act.PU

Sp7^{UASp.Tag:SS(ea)}

melanotic mass | P-stage, with Scer\GAL4^Act.PU

Sp7^{UASp.Tag:SS(ea)}

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (99)

Hsap\F10

3 of 14

Yes

Hsap\TPSAB1

3 of 14

Yes

Hsap\TPSB2

3 of 14

Yes

Hsap\TPSD1

3 of 14

Yes

Hsap\TPSG1

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\HABP2

1 of 14

Hsap\HGFAC

1 of 14

Hsap\HP

1 of 14

Yes

Hsap\HPR

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (91)

Rnor\F10

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

Rnor\C1r

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Masp2

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (99)

Mmus\Tpsab1

3 of 14

Yes

Mmus\Tpsb2

3 of 14

Yes

Mmus\Acr

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\C1rl

1 of 14

Yes

Mmus\Cela1

1 of 14

Mmus\Cela3a

1 of 14

Mmus\Cma2

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Masp2

1 of 14

Mmus\Mcpt2

1 of 14

Yes

Mmus\Mcpt4

1 of 14

Yes

Mmus\Mcpt9

1 of 14

Yes

Mmus\Mcptl

1 of 14

Yes

Mmus\Ovch2

1 of 14

Mmus\Pamr1

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (95)

Xtro\c1s

2 of 13

Yes

Xtro\cela1.2

2 of 13

Yes

Xtro\cfd

2 of 13

Yes

Xtro\f7

2 of 13

Yes

Xtro\f10

2 of 13

Yes

Xtro\gzma

2 of 13

Yes

Xtro\LOC548809

2 of 13

Yes

Xtro\LOC100491119

2 of 13

Yes

Xtro\LOC100495541

2 of 13

Yes

Xtro\LOC101731336

2 of 13

Yes

Xtro\LOC101734670

2 of 13

Yes

Xtro\LOC108647852

2 of 13

Yes

Xtro\LOC108648818

2 of 13

Yes

Xtro\LOC116407662

2 of 13

Yes

Xtro\LOC116407686

2 of 13

Yes

Xtro\LOC116411715

2 of 13

Yes

Xtro\pafah1b3

2 of 13

Yes

Xtro\proc.2

2 of 13

Yes

Xtro\prss8

2 of 13

Yes

Xtro\prss27

2 of 13

Yes

Xtro\prss29

2 of 13

Yes

Xtro\prss56

2 of 13

Yes

Xtro\tmprss3

2 of 13

Yes

Xtro\tmprss6

2 of 13

Yes

Xtro\tmprss13

2 of 13

Yes

Xtro\tpsg1

2 of 13

Yes

Xtro\XB5723326

2 of 13

Yes

Xtro\XB5892359

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\cela3a

1 of 13

Yes

Xtro\corin

1 of 13

Xtro\ctrc

1 of 13

Yes

Xtro\ctsg

1 of 13

Xtro\efcab7

1 of 13

Yes

Xtro\f2

1 of 13

Xtro\f12

1 of 13

Xtro\gprin3

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\LOC595077

1 of 13

Xtro\LOC100485189

1 of 13

Xtro\LOC100487305

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\LOC116412071

1 of 13

Yes

Xtro\LOC116412122

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Xtro\prss8l.5 loc108703873

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (79)

Drer\prss60.1

3 of 14

Yes

Drer\si:dkey-32n7.7

3 of 14

Yes

Drer\zgc:112038

3 of 14

Yes

Drer\zgc:123295

3 of 14

Yes

Drer\zgc:153968

3 of 14

Yes

Drer\zgc:163079

3 of 14

Yes

Drer\zgc:165423

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:ch73-182e20.4

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (13)

Cele\try-1

2 of 14

Yes

Cele\try-4

2 of 14

Yes

Cele\try-5

2 of 14

Yes

Cele\try-7

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (263)

Agam\CLIPB9

11 of 12

Yes

Agam\CLIPB1

5 of 12

Yes

4 of 12

4 of 12

4 of 12

4 of 12

4 of 12

4 of 12

Yes

4 of 12

4 of 12

4 of 12

4 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

Yes

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Agam\AgaP_AGAP004639

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Agam\AgaP_AGAP011430

1 of 12

Yes

Agam\AgaP_AGAP011590

1 of 12

Agam\AgaP_AGAP011668

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Agam\AgaP_AGAP012870

1 of 12

Agam\AgaP_AGAP013020

1 of 12

Agam\AgaP_AGAP013117

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Sp7. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (207)

7 of 13

6 of 13

5 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

MP1

4 of 13

SPE

4 of 13

Sph35

4 of 13

Sph242

4 of 13

tpr

4 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

flz

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

snk

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

mas

1 of 13

modSP

1 of 13

Phae2

1 of 13

psh

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

teq

1 of 13

Try29F

1 of 13

ζTry

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

F10; coagulation factor X

model of factor X deficiency

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

F10; coagulation factor X

3 of 14

613872

FACTOR X DEFICIENCY

factor X deficiency

TPSAB1; tryptase alpha/beta 1

3 of 14

191080

TPSB2; tryptase beta 2

3 of 14

191081

TPSD1; tryptase delta 1

3 of 14

609272

TPSG1; tryptase gamma 1

3 of 14

609341

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Sp7 - Hayan

enzymatic study, anti tag western blot

(Shan et al., 2023)

Sp7 - PPO1

enzymatic study, western blot

(Shan et al., 2023, An et al., 2013)

Sp7 - PPO2

enzymatic study, western blot

(Shan et al., 2023)

Sp7 - Spn27A

enzymatic study, western blot, comigration in gel electrophoresis

(An et al., 2013)

Sp7 - psh

enzymatic study, anti tag western blot

(Shan et al., 2023)

Sp7 - spz

enzymatic study, western blot

(Shan et al., 2023)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Sp7

suppressible

MP1

(Tang et al., 2006)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Fadd

enhanceable

Sp7

(Tang et al., 2006)

Spn77Ba

suppressible

Sp7

(Tang et al., 2008)

External Data

Subunit Structure (UniProtKB)

Interacts with Spn27A.

(UniProt, Q9V3Z2 )

Linkouts

BioGRID - A database of protein and genetic interactions.

66106

DroID - A comprehensive database of gene and protein interactions.

FBgn0037515

MIST (protein-protein) - An integrated Molecular Interaction Database

40918

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-48

Cytogenetic map

84D14-84E1

Sequence location

3R:7,759,786..7,763,947 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

84D14-84E1

Limits computationally determined from genome sequence between P{EP}EP3060^EP3060 and P{PZ}grn⁰⁵⁹³⁰

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-48

(FlyBase, 2016)

3-44.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (11)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

600361

w^*; Sp7^SK6, SPE^SK6

41612

y¹ v¹; P{TRiP.GL01194}attP40

13494

y¹ w^67c23; P{SUPor-P}Sp7^KG02818 ry⁵⁰⁶

51734

y¹ sc^* v¹ sev²¹; P{TRiP.HMS03162}attP2

44108

y¹ sc^* v¹ sev²¹; P{TRiP.HMS02829}attP40

NIG-Fly

3066R-1

P{NIG.3066R}1

3066R-3

P{NIG.3066R}3

HMS03162

y¹ sc^* v¹ sev²¹; P{TRiP.HMS03162}attP2

HMS02829

y¹ sc^* v¹ sev²¹; P{TRiP.HMS02829}attP40

VDRC

v104634

P{KK111500}VIE-260B

v1603

w¹¹¹⁸; P{GD468}v1603

Genomic Clones (26)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (12)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0037515

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0037515

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

RNAi of Sp7 reduces melanization at the wound site of infections, indicating that it is involved in the prophenoloxidase cascade.

(Kambris et al., 2006)

Relationship to Other Genes

Source for database merge of

Source for merge of: CG3066 anon-Ryu

(Bayraktaroglu, 2000.8.7)

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Sp7 CG3066

(Castillejo-Lopez and Häcker, 2005)

Source for identity of: Sp7 MP2

(Tang, 2006.2.28)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (20)

Reported As

Symbol Synonym

MP1

(Yu et al., 2022)

MP2

(Jin et al., 2023, Cao and Jiang, 2018, Veillard et al., 2016, Vlisidou and Wood, 2015, An et al., 2013, Kounatidis and Ligoxygakis, 2012, Nam et al., 2012, Scherfer et al., 2008, Tang et al., 2008, Bidla et al., 2007, Wu and Silverman, 2007, Tang, 2006.2.28, Tang et al., 2006, Tang et al., 2006)

MP2/sp7/PAE1

(Younes et al., 2020)

PAE

(Quintin et al., 2013)

PAE1

(Veillard et al., 2016, Vlisidou and Wood, 2015, Nam et al., 2012, Scherfer et al., 2008, Leclerc et al., 2006)

SP13

(Ross et al., 2003)

SP7

(Xu et al., 2023, Dudzic et al., 2019, Salazar-Jaramillo et al., 2014, Kambris et al., 2006, Ross et al., 2003)

Sp 7

(Park et al., 2014)

anon-Ryu

cSP7

(Cao and Jiang, 2018)

proPO-AE

(De Gregorio et al., 2002)

sp7

(Xu et al., 2016, Chambers et al., 2014, Winterhalter and Fedorka, 2009)

Name Synonyms

Melanization Protease 2

(Tang et al., 2006, Tang et al., 2006)

Serine protease 7

(Ordan and Volk, 2016, Xu et al., 2016, Clark et al., 2011)

Serine protease-7

(Castillo et al., 2015)

prophenoloxidase-activating enzyme

(Leclerc et al., 2006)

prophenoloxidase-activating enzyme 1

(Vlisidou and Wood, 2015)

serine protease 7

(Yadav et al., 2017, Marshall and Sinclair, 2010, Winterhalter and Fedorka, 2009)

Secondary FlyBase IDs

FBgn0040241

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 47 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40918

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9V3Z2

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9V3Z2

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9V3Z2

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40918/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0037515

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0037515

FlyMine - An integrated database for Drosophila genomics

FBgn0037515

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG3066

MARRVEL_MODEL - MARRVEL (model organism gene)

40918

Linkouts

BioGRID - A database of protein and genetic interactions.

66106

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0037515

DroID - A comprehensive database of gene and protein interactions.

FBgn0037515

DRSC - Results frm RNAi screens

FBgn0037515

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Sp7_1
Sp7_2

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0037515

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0037515

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3066

Flygut - An atlas of the Drosophila adult midgut

FBgn0037515

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC032162

MIST (protein-protein) - An integrated Molecular Interaction Database

40918

References (103)

The following external sites may use different assemblies or annotations than FlyBase.

-0.63

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