FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Sp7RNAi.UAS.WIZ
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General Information
Symbol
Dmel\Sp7RNAi.UAS.WIZ
Species
D. melanogaster
Name
FlyBase ID
FBal0197369
Feature type
allele
Associated gene
Dmel\Sp7
Associated Insertion(s)
Carried in Construct
P{UAS-Sp7.RNAi.WIZ}
Key Links
Transgenic product class
RNAi_reagent
(Tang et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Tang et al., 2006)
Mutagen
in vitro construct
(Tang et al., 2006)
Progenitor genotype
Carried in construct
P{UAS-Sp7.RNAi.WIZ}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat of approximately 600bp of Sp7 sequence.

(Tang et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Sp7
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies expressing Sp7dsRNA.Scer\UAS.WIZ under the control of Scer\GAL4da.G32 show no effect on mortality within 24 hours of mild wounding in 7-8 days old adults.

      Flies expressing Sp7dsRNA.Scer\UAS.WIZ under the control of Scer\GAL4Act5C.PI show comparable levels of mortality to wild type in response to mild wounding in 7-8 day old adults.

      (Nam et al., 2012)

      Approximately 50% of animals carrying Sp7dsRNA.Scer\UAS.WIZ and Scer\GAL4Act die at the late pupal stage or during eclosion. Adults expressing Sp7dsRNA.Scer\UAS.WIZ under the control of Scer\GAL4Act do not show melanisation around a wound site a few hours after infection by piercing with a tungsten needle harboring E.coli and M.luteus, in contrast to control flies which show significant melanisation around the wound site.

      Adult flies expressing Sp7dsRNA.Scer\UAS.WIZ under the control of Scer\GAL4da.G32 show a similar survival rate to control flies after bacterial (E.carotovora, E.faecalis) infection, although their survival is somewhat reduced compared to controls after natural fungal infection (B.bassiana).

      (Tang et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Suppressor of
      NOT Suppressor of
      Phenotype Manifest In
      Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of Sp7dsRNA.Scer\UAS.WIZ results in a further decrease in the survival rate of flies expressing DifdsRNA.IR.Scer\UAS under the control of Scer\GAL4da.G32 after fungal (C.albicans) infection.

      Co-expression of Sp7dsRNA.Scer\UAS.WIZ results in a moderate further decrease in the survival rate of flies expressing BG4dsRNA.Scer\UAS under the control of Scer\GAL4da.G32 after bacterial (E.carotovora) infection.

      (Tang et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      dsRNA is expressed in transgenic flies to study the phenotypic consequences of dsRNA interference (RNAi) of the Sp7 gene.

      (Tang et al., 2006)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Sp7RNAi.UAS.WIZ
      Sp7dsRNA.Scer\UAS.WIZ
      Sp7dsRNA.UAS.WIZ
      Name Synonyms
      Secondary FlyBase IDs
        References (4)