FlyBase Allele Report: Dmel\Spn77Ba[RNAi.WIZ.UAS]

General Information

Symbol

Dmel\Spn77Ba^RNAi.WIZ.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0244341

Feature type

allele

Associated gene

Dmel\Spn77Ba

Associated Insertion(s)

Carried in Construct

P{UAS-Spn77Ba.RNAi.WIZ}

Key Links

Transgenic product class

RNAi_reagent

(Tang et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Tang et al., 2008)

Mutagen

in vitro construct

(Tang et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-Spn77Ba.RNAi.WIZ}

Cytology

Description

UAS regulatory sequences drive expression of two inverted copies of a 551bp fragment corresponding to nucleotides 200-750 of Spn77Ba.

(Tang et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Spn77Ba

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of larval stage, with Scer\GAL4^Act.PU

(Tang et al., 2008)

some die during third instar larval stage, with Scer\GAL4^Act.PU

(Tang et al., 2008)

visible | adult stage, with Scer\GAL4^btl.PS

(Tang et al., 2008)

visible | larval stage, with Scer\GAL4^Act.PU

(Tang et al., 2008)

visible | larval stage, with Scer\GAL4^btl.PS

(Tang et al., 2008)

visible | pupal stage, with Scer\GAL4^btl.PS

(Tang et al., 2008)

Phenotype Manifest In

adult tracheal system, with Scer\GAL4^btl.PS

(Tang et al., 2008)

larval tracheal system, with Scer\GAL4^Act.PU

(Tang et al., 2008)

larval tracheal system, with Scer\GAL4^btl.PS

(Tang et al., 2008)

pupal tracheal system, with Scer\GAL4^btl.PS

(Tang et al., 2008)

tracheal section, with Scer\GAL4^da.G32

(Nam et al., 2012)

Detailed Description

Statement

Reference

Larvae expressing Spn77Ba^{dsRNA.WIZ.Scer\UAS} under the control of Scer\GAL4^da.G32 undergo spontaneous tracheal melanization.

(Nam et al., 2012)

Expression of Spn77Ba^{dsRNA.WIZ.Scer\UAS} under the control of Scer\GAL4^Act results in melanisation of the tracheal system in larvae. The phenotype is first visible during the second or third larval instar and increases in intensity until the affected larvae die within a few days before reaching the pupal stage.

Expression of Spn77Ba^{dsRNA.WIZ.Scer\UAS} under the control of Scer\GAL4^btl.PS results in tracheal melanisation in larvae, pupae and adults. The animals all develop to the prepupal stage. Approximately 35% of prepupae or pupae show severe melanisation of the tracheal system. The remaining 65% are able to develop into adults, of which 96% show tracheal melanisation.

81% of flies expressing Spn77Ba^{dsRNA.WIZ.Scer\UAS} under the control of Scer\GAL4^btl.PS are viable at 18[o]C, whereas all animals die before the adult stage if the animals also carry Spn77Ba^W7/+.

The melanisation resulting from expression of Spn77Ba^{dsRNA.WIZ.Scer\UAS} under the control of Scer\GAL4^btl.PS is first detected at a low level between tracheal cells and the overlying cuticle. As the amount of melanin increases, it spreads to encompass the epithelial layer occupied by the tracheal cells.

Flies expressing Spn77Ba^{dsRNA.WIZ.Scer\UAS} under the control of Scer\GAL4^c564 have a similar survival rate after microbial infection as control flies.

(Tang et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^btl.PS, Spn77Ba^RNAi.WIZ.UAS has lethal phenotype, enhanceable by MP1^{UASp.Tag:SS(ea)}, Scer\GAL4^btl.PS

(Tang et al., 2008)

Suppressed by

Statement

Reference

Scer\GAL4^Act.PU, Spn77Ba^RNAi.WIZ.UAS has lethal | larval stage phenotype, suppressible by Scer\GAL4^Act.PU/Sp7^RNAi.UAS.WIZ

(Tang et al., 2008)

Scer\GAL4^btl.PS, Spn77Ba^RNAi.WIZ.UAS has lethal phenotype, suppressible by MP1^RNAi.UAS.WIZ, Scer\GAL4^btl.PS

(Tang et al., 2008)

Scer\GAL4^btl.PS, Spn77Ba^RNAi.WIZ.UAS has lethal phenotype, suppressible by Scer\GAL4^btl.PS/Spn27A^UAS.cLa

(Tang et al., 2008)

Scer\GAL4^Act.PU, Spn77Ba^RNAi.WIZ.UAS has lethal | larval stage phenotype, suppressible by MP1^RNAi.UAS.WIZ, Scer\GAL4^Act.PU

(Tang et al., 2008)

NOT suppressed by

Statement

Reference

Scer\GAL4^btl.PS, Spn77Ba^RNAi.WIZ.UAS has lethal phenotype, non-suppressible by PPO1^Bc

(Tang et al., 2008)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^da.G32, Spn77Ba^RNAi.WIZ.UAS has tracheal section phenotype, suppressible by Hayan¹

(Nam et al., 2012)

Scer\GAL4^Act.PU, Spn77Ba^RNAi.WIZ.UAS has tracheal system phenotype, suppressible by MP1^RNAi.UAS.WIZ, Scer\GAL4^Act.PU

(Tang et al., 2008)

Scer\GAL4^Act.PU, Spn77Ba^RNAi.WIZ.UAS has tracheal system phenotype, suppressible by Scer\GAL4^Act.PU/Sp7^RNAi.UAS.WIZ

(Tang et al., 2008)

Scer\GAL4^btl.PS, Spn77Ba^RNAi.WIZ.UAS has adult tracheal system phenotype, suppressible by MP1^RNAi.UAS.WIZ, Scer\GAL4^btl.PS

(Tang et al., 2008)

Scer\GAL4^btl.PS, Spn77Ba^RNAi.WIZ.UAS has adult tracheal system phenotype, suppressible by Scer\GAL4^btl.PS/Spn27A^UAS.cLa

(Tang et al., 2008)

NOT suppressed by

Statement

Reference

Scer\GAL4^btl.PS, Spn77Ba^RNAi.WIZ.UAS has adult tracheal system phenotype, non-suppressible by PPO1^Bc

(Tang et al., 2008)

Scer\GAL4^btl.PS, Spn77Ba^RNAi.WIZ.UAS has adult tracheal system phenotype, non-suppressible by PGRP-SA^seml

(Tang et al., 2008)

Scer\GAL4^btl.PS, Spn77Ba^RNAi.WIZ.UAS has adult tracheal system phenotype, non-suppressible by psh⁵

(Tang et al., 2008)

Scer\GAL4^btl.PS, Spn77Ba^RNAi.WIZ.UAS has adult tracheal system phenotype, non-suppressible by spz⁴

(Tang et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

A Hayan¹ mutant background suppresses the spontaneous tracheal melanization seen when Spn77Ba^{dsRNA.WIZ.Scer\UAS} is expressed under the control of Scer\GAL4^da.G32.

(Nam et al., 2012)

Bc¹ does not suppress lethality or tracheal melanisation in adults caused by expression of Spn77Ba^{dsRNA.WIZ.Scer\UAS} under the control of Scer\GAL4^btl.PS.

The lethality and melanisation of the adult tracheal system caused by expression of Spn77Ba^{dsRNA.WIZ.Scer\UAS} under the control of Scer\GAL4^btl.PS is suppressed by co-expression of MP1^{dsRNA.Scer\UAS.WIZ}.

Co-expression of MP1^{Scer\UAS.P\T.T:ea} enhances the lethality caused by expression of Spn77Ba^{dsRNA.WIZ.Scer\UAS} under the control of Scer\GAL4^btl.PS.

Co-expression of Spn27A^Scer\UAS.cLa suppresses the lethality and tracheal melanisation caused by expression of Spn77Ba^{dsRNA.WIZ.Scer\UAS} under the control of Scer\GAL4^btl.PS.

The melanisation of the adult tracheal system caused by expression of Spn77Ba^{dsRNA.WIZ.Scer\UAS} under the control of Scer\GAL4^btl.PS is not suppressed by PGRP-SA^seml, psh⁵ or spz⁴.

The degree of melanisation seen in larvae expressing Spn77Ba^{dsRNA.WIZ.Scer\UAS} under the control of Scer\GAL4^btl.PS increases with greater microbial exposure.

(Tang et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Spn77Ba^RNAi.WIZ.UAS

Spn77Ba^{dsRNA.WIZ.Scer\UAS}

Spn77Ba^{dsRNA.WIZ.UAS}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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