FlyBase Allele Report: Dmel\MP1[RNAi.UAS.WIZ]

General Information

Symbol

Dmel\MP1^RNAi.UAS.WIZ

Species

D. melanogaster

Name

FlyBase ID

FBal0197366

Feature type

allele

Associated gene

Dmel\MP1

Associated Insertion(s)

Carried in Construct

P{UAS-MP1.RNAi.WIZ}

Key Links

Transgenic product class

RNAi_reagent

(Tang et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Tang et al., 2006)

Mutagen

in vitro construct

(Tang et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-MP1.RNAi.WIZ}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat of approximately 600bp of MP1 sequence.

(Tang et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

MP1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal wound healing, with Scer\GAL4^Act.PU

(Tang et al., 2006)

lethal, with Scer\GAL4^Act.PU

(Tang et al., 2006)

Phenotype Manifest In

Detailed Description

Statement

Reference

Cuticle melanisation induced by B.bassiana is partially suppressed by expression of MP1^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^Act.

(Tang et al., 2008)

Approximately 50% of animals carrying MP1^{dsRNA.Scer\UAS.WIZ} and Scer\GAL4^Act die at the late pupal stage or during eclosion. Adults expressing MP1^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^Act do not show melanisation around a wound site a few hours after infection by piercing with a tungsten needle harboring E.coli and M.luteus, in contrast to control flies which show significant melanisation around the wound site.

Adult flies expressing MP1^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^da.G32 show a similar survival rate to control flies after bacterial (E.carotovora, E.faecalis) and fungal (B.bassiana) infection.

(Tang et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

MP1^RNAi.UAS.WIZ, Scer\GAL4^da.G32 is an enhancer of abnormal immune response phenotype of Fadd^RNAi.UAS, Scer\GAL4^da.G32

(Tang et al., 2006)

Suppressor of

Statement

Reference

MP1^RNAi.UAS.WIZ, Scer\GAL4^Act.PU is a suppressor of lethal | larval stage phenotype of Scer\GAL4^Act.PU, Spn77Ba^RNAi.WIZ.UAS

(Tang et al., 2008)

MP1^RNAi.UAS.WIZ, Scer\GAL4^btl.PS is a suppressor of lethal phenotype of Scer\GAL4^btl.PS, Spn77Ba^RNAi.WIZ.UAS

(Tang et al., 2008)

MP1^RNAi.UAS.WIZ, Scer\GAL4^Act.PU is a suppressor of melanotic mass phenotype phenotype of Scer\GAL4^Act.PU, Sp7^{UASp.Tag:SS(ea)}

(Tang et al., 2006)

Phenotype Manifest In

Suppressor of

Statement

Reference

MP1^RNAi.UAS.WIZ, Scer\GAL4^Act.PU is a suppressor of tracheal system phenotype of Scer\GAL4^Act.PU, Spn77Ba^RNAi.WIZ.UAS

(Tang et al., 2008)

MP1^RNAi.UAS.WIZ, Scer\GAL4^btl.PS is a suppressor of adult tracheal system phenotype of Scer\GAL4^btl.PS, Spn77Ba^RNAi.WIZ.UAS

(Tang et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

The lethality and melanisation of the adult tracheal system caused by expression of Spn77Ba^{dsRNA.WIZ.Scer\UAS} under the control of Scer\GAL4^btl.PS is suppressed by co-expression of MP1^{dsRNA.Scer\UAS.WIZ}.

(Tang et al., 2008)

Co-expression of MP1^{dsRNA.Scer\UAS.WIZ} results in a further decrease in the survival rate of flies expressing Dif^{dsRNA.IR.Scer\UAS} under the control of Scer\GAL4^da.G32 after fungal (C.albicans) infection.

Co-expression of MP1^{dsRNA.Scer\UAS.WIZ} results in a moderate further decrease in the survival rate of flies expressing BG4^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 after bacterial (E.carotovora) infection.

(Tang et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

dsRNA is expressed in transgenic flies to study the phenotypic consequences of dsRNA interference (RNAi) of the MP1 gene.

(Tang et al., 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

MP1^RNAi.UAS.WIZ

MP1^{dsRNA.Scer\UAS.WIZ}

MP1^{dsRNA.UAS.WIZ}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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