FlyBase Allele Report: Dmel\Fadd[RNAi.UAS]

General Information

Symbol

Dmel\Fadd^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0138577

Feature type

allele

Associated gene

Dmel\Fadd

Associated Insertion(s)

Carried in Construct

P{UAS-Fadd.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Leulier et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Leulier et al., 2002)

Mutagen

in vitro construct

(Leulier et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-Fadd.RNAi}

Cytology

Description

Nucleotides 1-500 of the Fadd cDNA has been inserted as an inverted repeat and driven by UASt sequences.

(Leulier et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Fadd

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response, with Scer\GAL4^c564

(Kleino et al., 2005)

abnormal immune response, with Scer\GAL4^da.G32

(Tang et al., 2006)

abnormal immune response, with Scer\GAL4^yolk

(Leulier et al., 2002)

wild-type, with Scer\GAL4^da.G32

(Leulier et al., 2002)

Phenotype Manifest In

Detailed Description

Statement

Reference

Adult flies expressing BG4^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 show a reduced survival rate compared to control flies after bacterial (E.carotovora) infection.

(Tang et al., 2006)

The immune response to infection with the Gram-negative bacteria P.carotovorum, as determined by induction of Dpt, is reduced when BG4^{dsRNA.Scer\UAS} expression is driven in the fat body by Scer\GAL4^c564.

(Kleino et al., 2005)

When BG4^{dsRNA.Scer\UAS} is driven by Scer\GAL4^yolk, mutant animals are highly susceptible to Gram-negative bacterial infection, exhibiting a dramatic loss of survival rate after infection, with almost all animals dying within 4 days. However, mutants appear resistant to fungal infection. Expression of BG4^{dsRNA.Scer\UAS} when driven by Scer\GAL4^da.G32 shows no detectable developmental defects.

(Leulier et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Fadd^RNAi.UAS, Scer\GAL4^da.G32 has abnormal immune response phenotype, enhanceable by MP1^RNAi.UAS.WIZ, Scer\GAL4^da.G32

(Tang et al., 2006)

Fadd^RNAi.UAS, Scer\GAL4^da.G32 has abnormal immune response phenotype, enhanceable by Sp7^RNAi.UAS.WIZ, Scer\GAL4^da.G32

(Tang et al., 2006)

Suppressor of

Statement

Reference

Fadd^RNAi.UAS, Scer\GAL4^Switch1.106 is a suppressor of abnormal immune response phenotype of PGRP-LE^UAS.Tag:FLAG, Scer\GAL4^Switch1.106

(Libert et al., 2006)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Simultaneous expression of BG4^{dsRNA.Scer\UAS} driven by Scer\GAL4^S106-GS significantly suppresses the increased pathogen resistance phenotype of PGRP-LE^{Scer\UAS.T:Zzzz\FLAG}-expressing flies.

(Libert et al., 2006)

Co-expression of either MP1^{dsRNA.Scer\UAS.WIZ} or Sp7^{dsRNA.Scer\UAS.WIZ} results in a moderate further decrease in the survival rate of flies expressing BG4^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 after bacterial (E.carotovora) infection.

(Tang et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Transcription from the P{UAS-BG4-dsRNA} construct should produce BG4 dsRNA, resulting in dsRNA interference (RNAi) of the BG4 gene.

(Leulier et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

BG4^{dsRNA.Scer\UAS}

Fadd^RNAi.UAS

Fadd^{dsRNA.Scer\UAS}

Fadd^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

Open Close