FB2021_02, released April 13, 2021

Allele: Dmel\Spn27AUAS.cLa

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General Information
Symbol
Dmel\Spn27AUAS.cLa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Ligoxygakis
FlyBase ID
FBal0144538
Feature type
allele
Associated gene
Dmel\Spn27A
Associated Insertion(s)
Carried in Construct
P{UAS-Spn27A.L}
Also Known As
UAS-Spn27A
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - regulatory fusion
(Ligoxygakis et al., 2002)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{UAS-Spn27A.L}
      Cytology
      Nature of the lesion
      Statement
      Reference

      UASt sequences drive expression of Spn27A.

      (Ligoxygakis et al., 2002)
      Allele components
      Product class / Tool use(s)
      Regulatory region(s)
      UASt
      Encoded product / tool
      Spn27A
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies expressing Spn27AScer\UAS.cLa under the control of Scer\GAL4Act5C show a strongly reduced melanisation reaction at a wound site 16 hours after septic injury compared to control flies.

      (Scherfer et al., 2008)

      Expression of Spn27AScer\UAS.cLa ubiquitously under the control of Scer\GAL4da.G32, or in crystal cells under the control of Scer\GAL4lz-gal4, completely inhibits melanization of hemolymph clots.

      (Bidla et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      Scer\GAL4btl.PS/Spn27AUAS.cLa is a suppressor of lethal phenotype of Scer\GAL4btl.PS, Spn77BadsRNA.WIZ.UAS

      (Tang et al., 2008)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of Spn27AScer\UAS.cLa suppresses the lethality and tracheal melanisation caused by expression of Spn77BadsRNA.WIZ.Scer\UAS under the control of Scer\GAL4btl.PS.

      (Tang et al., 2008)

      Expression of Spn27AScer\UAS.cLa in larval hemocytes expressing grimScer\UAS.cNa under the control of Scer\GAL4He.PZ results in almost no melanization.

      (Bidla et al., 2007)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4da.G32/Spn27AUAS.cLa rescues Spn27Aex32

      (Ligoxygakis et al., 2002)
      Comments

      The addition of Spn27AScer\UAS.cLa (driven by Scer\GAL4da.G32) suppresses both lethality and melanisation phenotypes seen in Spn27Aex32.

      (Ligoxygakis et al., 2002)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Spn27AScer\UAS.cLa
      Spn27AUAS.cLa
      Spn27AUAS
      (Ligoxygakis et al., 2003)
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Ligoxygakis
      (Ligoxygakis et al., 2002)
      Secondary FlyBase IDs
        References (7)