FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Allele: Dmel\Spn27Aex32
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General Information
Symbol
Dmel\Spn27Aex32
Species
D. melanogaster
Name
FlyBase ID
FBal0144536
Feature type
allele
Associated gene
Dmel\Spn27A
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
Delta2-3 transposase
(Ligoxygakis et al., 2002)
Progenitor genotype
Spn27Ad03636
(Ligoxygakis et al., 2002)
Cytology
Description

A 1.4 kb deletion removes 1.2kb of the open reading frame of Spn27A.

(Ligoxygakis et al., 2002)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Embryos derived from Spn27Aex32/Df(2L)BSC7 females completely lack cuticle, since all cells adopt a mesodermal fate.

      (Ligoxygakis et al., 2003)

      About 40% of homozygous mutant or Spn27Aex32/Df(2L)BSC7 larvae, and 35% of mutant adults exhibit a constitutive melanisation phenotype. Melanisation is particularly conspicuous around internal organs such as gut and fat body, but is never associated with barrier epithelia of the body wall. Most of the mutant larvae that develop spontaneous melanisation die in mid-pupal stages. Survival of Spn27Aex32 homozygous adults following bacterial challenge with Gram-negative or positive bacterial or fungal infection is comparable to wild-type. However mutants are particularly sensitive in term as of their melanisation reaction to injury-couple infection, as this treatment results in the appearance of extended melanisation around the wound.

      (Ligoxygakis et al., 2002)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Embryos derived from Spn27Aex32/Df(2L)BSC7 ; pip1/pip2 females are completely dorsalised. Embryos derived from Spn27Aex32/Df(2L)BSC7 ; snk1/snk2 females are completely dorsalised. Embryos derived from Spn27Aex32/Df(2L)BSC7 ; ea1/ea2 females are completely dorsalised. Embryos derived from Spn27Aex32/Df(2L)BSC7 ; spz4/spz4 females are completely dorsalised. Embryos derived from gd9/gd9 ; Spn27Aex32/Df(2L)BSC7 females express either a dorsolateral or ventrolateral fate around their circumference.

      (Ligoxygakis et al., 2003)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Spn27Aex32 is rescued by Scer\GAL4da.G32/Spn27AUAS.cLa

      (Ligoxygakis et al., 2002)
      Comments

      Injection of Spn27AcLa RNA into pre-blastoderm embryos derived from Spn27Aex32/Df(2L)BSC7 females can restore the wild-type cuticle pattern or cause a mild dorsalisation.

      (Ligoxygakis et al., 2003)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Spn27Aex32
      spn27Aex
      (Gomez et al., 2024)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)