y promoter sequences at the endogenous locus have been replaced with heterologous Hsp70 promoter sequences by targeted gene replacement at yh12w+. In addition, a loxP cassette containing a 593bp fragment that contains the entire gypsy\su(Hw)BR insulator has been inserted 700bp upstream of the transcription start site, in between the endogenous y wing and body enhancers and the heterologous promoter.
Body colour: pigmentation score (where 1 = null/nearly null, 5 = wild type/nearly wild type): hemizygote: 5 (wing), 4+-5 (body), yP1\loxP.hsp70-700gin/y1 : 5 (wing), 4+-5 (body), yP1\loxP.hsp70-700gin/y1#8 : 5 (wing), 5+ (body), yP1\loxP.hsp70-700gin/y59b : 5 (wing), 5 (body), yP1\loxP.hsp70-700gin/y3c3 : 5 (wing), 5+ (body).