FlyBase Allele Report: Dmel\GLaz[UAS.cWa]

General Information

Symbol

Dmel\GLaz^UAS.cWa

Species

D. melanogaster

Name

FlyBase ID

FBal0193460

Feature type

allele

Associated gene

Dmel\GLaz

Associated Insertion(s)

Carried in Construct

P{UAS-Glaz.W}

Also Known As

UAS-Glaz, UAS:GLaz

Key Links

Transgenic product class

wild_type

(Walker et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Walker et al., 2006)

Mutagen

in vitro construct

(Walker, 2006, Walker et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-Glaz.W}

Cytology

Description

UASt regulatory sequences drive expression of GLaz coding sequences.

(Walker et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

GLaz

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Friedreich ataxia

modeled by fh^RNAi.UAS.cAa

(Navarro et al., 2010)

modeled by fh^RNAi.UAS.cLa

(Navarro et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal stress response, with Scer\GAL4^109(2)80

(Walker, 2006, Walker et al., 2006)

abnormal stress response, with Scer\GAL4^da.G32

(Walker et al., 2006)

increased rate of movement, with Scer\GAL4^109(2)80

(Walker et al., 2006)

long lived, with Scer\GAL4^109(2)80

(Walker, 2006, Walker et al., 2006)

long lived | heat sensitive, with Scer\GAL4^12.1

(Walker et al., 2006)

long lived | heat sensitive, with Scer\GAL4^109(2)80

(Walker et al., 2006)

long lived | heat sensitive, with Scer\GAL4^GMR.PF

(Walker et al., 2006)

long lived | heat sensitive, with Scer\GAL4^how-24B

(Walker et al., 2006)

long lived | heat sensitive, with Scer\GAL4^Lk6-DJ634

(Walker et al., 2006)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of GLaz^Scer\UAS.cWa, under the control of Scer\GAL4^D42, Scer\GAL4^Act5C, Scer\GAL4^hs.PB or Scer\GAL4^elav-C155 at 29^oC does not have any significant effect on lifespan.

Expression of GLaz^Scer\UAS.cWa in the central nervous system and some muscles, under the control of Scer\GAL4^12.1 results in an 8% increase in lifespan.

Expression of GLaz^Scer\UAS.cWa in the central nervous system and some muscles, under the control of Scer\GAL4^109(2)80 at 29^oC results in an 23% increase in lifespan.

Expression of GLaz^Scer\UAS.cWa in the fat and some muscles, under the control of Scer\GAL4^Lk6-DJ634 at 29^oC results in an 16% increase in lifespan.

Expression of GLaz^Scer\UAS.cWa in the fat and some muscles, under the control of Scer\GAL4^how-24B at 29^oC results in an 28% increase in lifespan.

Expression of GLaz^Scer\UAS.cWa in the eye and salivary glands, under the control of Scer\GAL4^GMR.PF at 29^oC results in an 23% increase in lifespan.

Flies carrying both GLaz^Scer\UAS.cWa and Scer\GAL4^109(2)80 at 29^oC display a 12% increase in survival under hyperoxia compared to the Scer\GAL4^109(2)80 driver control.

After 4 days in hyperoxia, flies carrying both GLaz^Scer\UAS.cWa and Scer\GAL4^109(2)80 at 29^oC display both vertical and horizontal locomotor performances that are much improved compared to controls. No differences are apparent under normoxia.

Overexpression of GLaz^Scer\UAS.cWa under the control of Scer\GAL4^109(2)80 leads to a 60% increase in lifespan under wet starvation and a 30% increase under dry starvation. There is no significant difference in dry weight, protein content, or fat content between GLaz^Scer\UAS.cWa Scer\GAL4^109(2)80 flies and controls.

Flies overexpressing GLaz^Scer\UAS.cWa under the control of Scer\GAL4^109(2)80 that are subjected to hypoxia in 100% N₂ for 30 minutes, then transferred back to a normal environment are much more active than control flies. One hour after recovery, approximately 75% of the overexpression flies climb up to the top third of the vial, while only 10-15% of control flies manage the same. This difference is still evident 2 days later. There is no difference in climbing ability before hypoxia exposure.

(Walker et al., 2006)

(Walker, 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

GLaz^UAS.cWa, Scer\GAL4^repo.PU is a suppressor | partially of abnormal oxidative stress response | adult stage phenotype of Scer\GAL4^repo.PU, fh^RNAi.UAS.cAa

(Navarro et al., 2010)

GLaz^UAS.cWa, Scer\GAL4^repo.PU is a suppressor | partially of short lived phenotype of Scer\GAL4^repo.PU, fh^RNAi.UAS.cAa

(Navarro et al., 2010)

GLaz^UAS.cWa, Scer\GAL4^repo.PU is a suppressor | partially of abnormal locomotor behavior phenotype of Scer\GAL4^repo.PU, fh^RNAi.UAS.cAa

(Navarro et al., 2010)

GLaz^UAS.cWa, Scer\GAL4^repo.PU is a suppressor | partially of abnormal oxidative stress response | adult stage phenotype of Scer\GAL4^repo.PU, fh^RNAi.UAS.cLa

(Navarro et al., 2010)

GLaz^UAS.cWa, Scer\GAL4^repo.PU is a suppressor | partially of short lived phenotype of Scer\GAL4^repo.PU, fh^RNAi.UAS.cLa

(Navarro et al., 2010)

GLaz^UAS.cWa, Scer\GAL4^repo.PU is a suppressor | partially of abnormal locomotor behavior phenotype of Scer\GAL4^repo.PU, fh^RNAi.UAS.cLa

(Navarro et al., 2010)

Other

Statement

Reference

GLaz^UAS.cWa, Scer\GAL4^repo.PU, fh^RNAi.UAS.cAa has abnormal neuroanatomy | adult stage phenotype

(Navarro et al., 2010)

Phenotype Manifest In

Other

Statement

Reference

GLaz^UAS.cWa, Scer\GAL4^repo.PU, fh^RNAi.UAS.cAa has adult CNS glial cell phenotype

(Navarro et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of GLaz^Scer\UAS.cWa changes the quality of accumulated lipid droplets in brain glial cells of adult flies expressing fh^{dsRNA.IR.Scer\UAS} under the control of Scer\GAL4^repo.PU.

The reduced lifespan, decreased survival under hyperoxia conditions as well as the impaired climbing ability characteristic for adult flies expressing either fh^{dsRNA.IR.Scer\UAS} or fh^{dsRNA.Scer\UAS.cLa} under the control of Scer\GAL4^repo.PU is partially restored by co-expression of GLaz^Scer\UAS.cWa.

(Navarro et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

600611

w^*; P{UAS-Glaz.W}2

27905

w^*; P{UAS-Glaz.W}2; P{lacW}SNF4Aγ^loe/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

GLaz^Scer\UAS.cWa

GLaz^UAS.cWa

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

Open Close