FlyBase Allele Report: Dmel\Sxl[M3.UAS]

General Information

Symbol

Dmel\Sxl^M3.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0193570

Feature type

allele

Associated gene

Dmel\Sxl

Associated Insertion(s)

Carried in Construct

P{UAS-Sxl.M3}

Key Links

Transgenic product class

cDNA

(Horabin, 2005)

wild_type

(Horabin, 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Horabin, 2005)

wild_type

(Horabin, 2005)

Mutagen

in vitro construct

(Horabin, 2005)

Progenitor genotype

Carried in construct

P{UAS-Sxl.M3}

Cytology

Description

The M3 isoform of Sxl is placed downstream of UAS regulatory sequences.

(Horabin, 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Sxl

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

hyperplasia | larval stage, with Scer\GAL4^dpp.blk1

(Horabin, 2005)

increased occurrence of cell division | cell non-autonomous | larval stage, with Scer\GAL4^dpp.blk1

(Horabin, 2005)

increased occurrence of cell division | larval stage, with Scer\GAL4^ap-md544

(Horabin, 2005)

increased occurrence of cell division | larval stage, with Scer\GAL4^dpp.blk1

(Horabin, 2005)

Phenotype Manifest In

wing disc, with Scer\GAL4^ap-md544

(Horabin, 2005)

wing disc, with Scer\GAL4^dpp.blk1

(Horabin, 2005)

Detailed Description

Statement

Reference

Expression of Sxl^M3.Scer\UAS, in the wing disc, under the control of Scer\GAL4^ap-md544 results in an elevated mitotic index (as illustrated by BrdU and phospho-His3 staining).

Expression of Sxl^M3.Scer\UAS under the control of Scer\GAL4^dpp.blk1 results in wing disc overgrowth, not only in the anterior compartment, but also in the posterior compartment, indicating a non-autonomous function.

(Horabin, 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Scer\GAL4^ap-md544, Sxl^M3.UAS, hh^C84S.UAS has lethal | pupal stage phenotype

(Horabin, 2005)

Phenotype Manifest In

Other

Statement

Reference

Scer\GAL4^ap-md544, Sxl^M3.UAS, hh^C84S.UAS has wing phenotype

(Horabin, 2005)

Scer\GAL4^ap-md544, Sxl^M3.UAS, hh^C84S.UAS has wing disc phenotype

(Horabin, 2005)

Scer\GAL4^ap-md544, Sxl^M3.UAS, hh^C84S.UAS has wing cell phenotype

(Horabin, 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Male and female wing discs, when expressing Sxl^M3.Scer\UAS and hh^{C84S.Scer\UAS} under the control of Scer\GAL4^dpp.blk1 show a change in their normal proportions that is suggestive of enlargement. All male discs show the posterior en signal with an anterior extension, with the rest of the anterior compartment relatively normal in size. In females, the anterior compartment is larger than the posterior, frequently by almost twofold.

Co-expressing Sxl^M3.Scer\UAS and hh^{C84S.Scer\UAS} under the control of Scer\GAL4^dpp.blk1 is essentially lethal to both males and females; rare female escapers have misshapen wings with bubbles and a narrowing of the L3 and L4 intervein region (this is also observed in hh^{C84S.Scer\UAS}-expressing flies).

(Horabin, 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Sxl^M3.Scer\UAS

Sxl^M3.UAS

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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