A heat shock promoter drives expression of a fusion protein composed of the DNA-binding domain of Scer\GAL4 (amino acid residues 1-145), a Tag:MYC epitope and Tag:Dam.
Carried in a plasmid, transfected into Kc167 cells, and used in DamID assays to map in vivo genomic binding sites.
Carried in a plasmid, transfected into Kc167 cells, and used in DamID assays to map in vivo genomic binding sites.