FlyBase Allele Report: Dmel\Camta[tes-2]

General Information

Symbol

Dmel\Camta^tes-2

Species

D. melanogaster

Name

termination slow²

FlyBase ID

FBal0194678

Feature type

allele

Associated gene

Dmel\Camta

Associated Insertion(s)

Carried in Construct

Also Known As

tes²

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Han et al., 2006)

Progenitor genotype

Cytology

Description

Amino acid replacement: S577R.

(Han et al., 2006)

Amino acid replacement: L1420term.

(Han et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:9,471,547..9,471,547 [+]

Nucleotide change:

A9471547C

Amino acid change:

Reported amino acid change:

S577R

Comment:

Reported mutation locations are relative to GB:DQ902587. A nucleotide change at the first or third position of the Ser codon leads to an Arg mutation (base change unspecified).

(Han et al., 2006)

point_mutation

2R:9,474,419..9,474,419 [+]

Nucleotide change:

T9474419A

Amino acid change:

Reported amino acid change:

L1420term

Comment:

Reported mutation locations are relative to GB:DQ902587. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Han et al., 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology

(Han et al., 2006)

Phenotype Manifest In

eye photoreceptor cell

(Han et al., 2006)

Detailed Description

Statement

Reference

Electroretinogram (ERG) responses terminate slowly in Camta^tes-2 flies compared to wild-type flies. Whole-cell recordings of isolated Camta^tes-2 photoreceptor cells reveal a defective termination of light response.

The rhabdomeres of 1-day-old Camta^tes-2 flies show normal morphology.

Quantum bumps in wild-type and Camta^tes-2 photoreceptors have similar shapes and amplitude distributions. However, Camta^tes-2 mutant cells generate multiple bumps when stimulated with a flash of dim light, while wild-type cells produce only a single bump.

The minimum light intensity needed to induce prolonged depolarization afterpotentials is ~600 lux in Camta^tes-2 flies, while ~2600 lux is needed to induce this in wild-type flies.

(Han et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Camta^tes-2 has abnormal neurophysiology phenotype, enhanceable by Arr2⁵

(Han et al., 2006)

Suppressed by

Statement

Reference

Camta^tes-2 has abnormal neurophysiology phenotype, suppressible by Fbxl4^trp.PH

(Han et al., 2006)

Camta^tes-2 has abnormal neurophysiology phenotype, suppressible by Fbxl4^hs.PH

(Han et al., 2006)

Camta^tes-2 has abnormal neurophysiology phenotype, suppressible by Gαq¹

(Han et al., 2006)

NOT suppressed by

Statement

Reference

Camta^tes-2 has abnormal neurophysiology phenotype, non-suppressible by trp^Δ1272

(Han et al., 2006)

Camta^tes-2 has abnormal neurophysiology phenotype, non-suppressible by Cpr72Ec^hs.PH

(Han et al., 2006)

Camta^tes-2 has abnormal neurophysiology phenotype, non-suppressible by ltl^hs.PH

(Han et al., 2006)

Enhancer of

Statement

Reference

Camta^tes-2 is an enhancer of abnormal neurophysiology phenotype of Arr2⁵

(Han et al., 2006)

Other

Statement

Reference

Camta^tes-2, ninaE⁷ has abnormal neurophysiology phenotype

(Han et al., 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

Camta^tes-2 has eye photoreceptor cell phenotype, enhanceable by Arr2⁵

(Han et al., 2006)

Suppressed by

Statement

Reference

Camta^tes-2 has eye photoreceptor cell phenotype, suppressible by Gαq¹

(Han et al., 2006)

Camta^tes-2 has eye photoreceptor cell phenotype, suppressible by Fbxl4^trp.PH

(Han et al., 2006)

Camta^tes-2 has eye photoreceptor cell phenotype, suppressible by Fbxl4^hs.PH

(Han et al., 2006)

NOT suppressed by

Statement

Reference

Camta^tes-2 has eye photoreceptor cell phenotype, non-suppressible by trp^Δ1272

(Han et al., 2006)

Camta^tes-2 has eye photoreceptor cell phenotype, non-suppressible by Cpr72Ec^hs.PH

(Han et al., 2006)

Camta^tes-2 has eye photoreceptor cell phenotype, non-suppressible by ltl^hs.PH

(Han et al., 2006)

Enhancer of

Statement

Reference

Camta^tes-2 is an enhancer of eye photoreceptor cell phenotype of Arr2⁵

(Han et al., 2006)

Other

Statement

Reference

Camta^tes-2, ninaE⁷ has eye photoreceptor cell phenotype

(Han et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The Gα49B¹ mutation suppresses the multiple bump phenotype seen in Camta^tes-2 mutant photoreceptors following stimulation with a flash of dim light.

The trp^Δ1272 mutation does not improve the termination response of Camta^tes-2 photoreceptors.

The termination photoresponses of Camta^tes-2; ninaE⁷ double mutant flies are as fast as wild-type flies, but the amplitude of the responses is decreased. In Camta^tes-2; ninaE⁷/+ flies, the response amplitude is similar to wild-type, but the termination response is increased compared to Camta^tes-2 single mutant flies.

The termination speed of photoresponses in Camta^tes-2; Arr2⁵ double mutants is much slower than those of either single mutant.

Expression of either Fbxl4^trp.PH or Fbxl4^hs.PH rescues the slow-termination phenotype of Camta^tes-2 photoreceptors.

Expression of either CG4784^hs.PH or CG32372^hs.PH has no effect the Camta^tes-2 mutant phenotype.

(Han et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Camta^tes-1

(Han et al., 2006)

Rescued by

Camta^tes-2 is rescued by Camta^trp.PH

(Han et al., 2006)

Camta^tes-2 is rescued by Camta^hs.PH

(Han et al., 2006)

Camta^tes-2 is rescued by Camta^m2Q1.trp

(Han et al., 2006)

Camta^tes-2 is rescued by Camta^m2Q1.hs

(Han et al., 2006)

Camta^tes-2 is rescued by Camta^m3Q1.trp

(Han et al., 2006)

Camta^tes-2 is rescued by Camta^m3Q1.hs

(Han et al., 2006)

Partially rescued by

Camta^tes-2 is partially rescued by Camta^mlQ1.trp

(Han et al., 2006)

Camta^tes-2 is partially rescued by Camta^mlQ1.hs

(Han et al., 2006)

Comments

Expression of Camta^hs.PH, following a heat shock, results in a full rescue of the Camta^tes-2 phenotype. However, although high levels of Camta protein are seen at 1 hour following heat shock, full rescue is only achieved following 10 hours.

(Han et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: a mutation that affects phototransduction in an electroretinogram (ERG)-based screen.

(Han et al., 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Camta^tes-2

Camta^tes2

(Mishra et al., 2016)

tes²

(Vuong-Brender et al., 2021, Han et al., 2006)

Name Synonyms

termination slow2

Secondary FlyBase IDs

References (3)

Report Sections

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