FlyBase Allele Report: Dmel\Nrg[RNAi.UAS]

General Information

Symbol

Dmel\Nrg^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0194844

Feature type

allele

Associated gene

Dmel\Nrg

Associated Insertion(s)

Carried in Construct

P{UAS-Nrg.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Yamamoto, 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Yamamoto, 2006)

Mutagen

in vitro construct

(Yamamoto, 2006)

Progenitor genotype

Carried in construct

P{UAS-Nrg.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat of Nrg cDNA, corresponding to amino acid residues 1-166.

(Yamamoto, 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Nrg

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^pk-IG1-1, Scer\GAL4^repo

(Yamamoto et al., 2006)

abnormal neuroanatomy, with Scer\GAL4^repo

(Yamamoto, 2006)

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav-C155

(Yang et al., 2011)

Phenotype Manifest In

dendrite | larval stage, with Scer\GAL4^elav-C155

(Yang et al., 2011)

dendritic arborizing neuron | larval stage, with Scer\GAL4^elav-C155

(Yang et al., 2011)

dorsal multidendritic neuron ddaD & dendritic tree, with Scer\GAL4^repo

(Yamamoto, 2006)

dorsal multidendritic neuron ddaE & axon, with Scer\GAL4^pk-IG1-1, Scer\GAL4^repo

(Yamamoto et al., 2006)

dorsal multidendritic neuron ddaE & axon, with Scer\GAL4^repo

(Yamamoto, 2006)

Detailed Description

Statement

Reference

The number of dendritic endpoints of dorsal dendritic arborisation neurons is reduced compared to controls in larvae expressing Nrg^{dsRNA.Scer\UAS} under the control of Scer\GAL4^elav-C155.

(Yang et al., 2011)

Expression of Nrg^{dsRNA.Scer\UAS} in neurons and glia, driven by Scer\GAL4^IG1-1 and Scer\GAL4^repo (resulting in the production of Nrg dsRNA) in embryos does not causes significant branching of the ddaE neuron. This phenotype is not seen when only Scer\GAL4^IG1-1 is used to drive expression of Nrg^{dsRNA.Scer\UAS}.

(Yamamoto et al., 2006)

Expression of Nrg^{dsRNA.Scer\UAS} in glia, driven by Scer\GAL4^repo, (leading to production of Nrg dsRNA) results in the termination of glial processes midway on axons to ddaE cell bodies and the exposure of ddaE axonal roots.

(Yamamoto, 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Transcription from the P{UAS-Nrg.RNAi} construct should produce Nrg dsRNA, resulting in dsRNA interference (RNAi) of the Nrg gene.

(Yamamoto, 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Nrg^RNAi.UAS

Nrg^{dsRNA.Scer\UAS}

Nrg^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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