FlyBase Allele Report: Dmel\dj-1β[ex54]

General Information

Symbol

Dmel\dj-1β^ex54

Species

D. melanogaster

Name

FlyBase ID

FBal0195124

Feature type

allele

Associated gene

Dmel\dj-1β

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Park et al., 2005)

Mutagen

Delta2-3 transposase

(Park et al., 2005)

Progenitor genotype

dj-1β^GE23381

(Park et al., 2005)

Cytology

Description

Imprecise excision of the dj-1β^GE23381 P-element resulted in an approximately 450 bp deletion, removing the first two exons and part of the third exon including the translation start site.

(Park et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Parkinson's disease 7

CEA

(Lee et al., 2022, Park et al., 2005)

CEC

(Kim et al., 2018)

model of Parkinson's disease

CEA

(Hwang et al., 2013)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of Parkinson's disease 7

is ameliorated by Pdk^UAS.Tag:HA

(Lee et al., 2022)

is ameliorated by Pdp^G1628

(Lee et al., 2022)

is ameliorated by Vhl¹

(Lee et al., 2022)

is ameliorated by sima^UAS.cBa

(Lee et al., 2022)

is ameliorated by Cyp1^EP1073

(Kim et al., 2018)

model of Parkinson's disease

is ameliorated by Daxx¹

(Hwang et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

dj-1β^ex54 mutants model Parkinson's disease under oxidative stress conditions.

(Hwang et al., 2013)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage

(Hwang et al., 2013)

abnormal locomotor behavior | recessive

(Park et al., 2005)

abnormal oxidative stress response | adult stage

(Hwang et al., 2013)

abnormal oxidative stress response | larval stage

(Hwang et al., 2013)

fertile

(Park et al., 2005)

radiation sensitive

(Hwang et al., 2013)

viable

(Park et al., 2005)

Phenotype Manifest In

dopaminergic neuron | adult stage

(Hwang et al., 2013)

dorso-lateral dopaminergic neuron

(Hwang et al., 2013)

larval brain

(Hwang et al., 2013)

Detailed Description

Statement

Reference

Mutant adults show a significant decrease in the number of dorsomedial cluster, dorsolateral cluster 1 and posteriomedial cluster dopaminergic neurons in the brain under oxidative stress conditions compared to wild-type flies.

Mutant flies show reduced survival compared to controls when fed with hydrogen peroxide.

Oxidative stress conditions result in a large increase in apoptosis in the brains of mutant larvae compared to the level seen in wild-type controls.

Mutants show reduced survival after UV irradiation compared to wild type.

Five day old flies show reduced climbing ability compared to wild type.

(Hwang et al., 2013)

dj-1β^ex54 mutants do not display any external phenotypic defects.

The morphology of dopaminergic neurons in dj-1β^ex54 mutant brains show no obvious abnormalities, and the neuronal axons seem normally projected as compared to age-matched wild type controls, even at 30 days of age.

The number of dopaminergic neurons in dj-1β^ex54 mutant brains is normal and there is no evidence of Lewy body formation.

The climbing ability of dj-1β^ex54 mutants is reduced to about half of that of wild type even on the first day of eclosion, and almost completely lost after 30 days, indicating that dj-1β^ex54 mutants show a premature loss of climbing ability. The declining rate over time in dj-1β^ex54 mutants and wild type are similar though.

(Park et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

dj-1β^ex54 has abnormal oxidative stress response | adult stage phenotype, suppressible by Daxx¹

(Hwang et al., 2013)

dj-1β^ex54 has abnormal oxidative stress response | larval stage phenotype, suppressible by Daxx¹

(Hwang et al., 2013)

dj-1β^ex54 has abnormal oxidative stress response | adult stage phenotype, suppressible by Daxx²

(Hwang et al., 2013)

dj-1β^ex54 has radiation sensitive phenotype, suppressible by Daxx¹

(Hwang et al., 2013)

dj-1β^ex54 has radiation sensitive phenotype, suppressible by Daxx²

(Hwang et al., 2013)

dj-1β^ex54 has abnormal locomotor behavior | adult stage phenotype, suppressible by Daxx¹

(Hwang et al., 2013)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The reduced survival and reduction in number of dopaminergic neurons in the brain seen in dj-1β^ex54 adults under oxidative stress conditions are suppressed if the animals are also mutant for DLP¹.

The reduced survival seen in dj-1β^ex54 adults under oxidative stress conditions are suppressed if the animals are also mutant for DLP².

The increased apoptosis seen in the brains of dj-1β^ex54 larvae under oxidative stress conditions is suppressed if the animals are also mutant for DLP¹.

The sensitivity to UV irradiation seen in dj-1β^ex54 animals is suppressed if they are also mutant for either DLP¹ or DLP².

The reduced climbing ability of dj-1β^ex54 flies is suppressed by DLP¹.

(Hwang et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

DJ-1β^ex54

(Lee et al., 2022, Solana-Manrique et al., 2022, Sanz et al., 2021, Solana-Manrique et al., 2021, Solana-Manrique et al., 2020, Kim et al., 2018, Poudel and Lee, 2018, Yang et al., 2017, Hwang et al., 2013, Park et al., 2005)

dj-1β^ex54

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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