Animals expressing LIMK1^{ΔNgoMIV.Scer\UAS} under the control of Scer\GAL4^ap-md544 show a temperature-sensitive phenotype. At 28^oC, all progeny die, at 25^oC most animals die as pupae (rare escapers at this temperature have severe wing defects), and at 23^oC, the progeny are fully viable.

Scer\GAL4^ap-md544>LIMK1^{ΔNgoMIV.Scer\UAS} flies raised at 23^oC show planar polarity defects; the majority of these flies show a chaotic wing hair orientation pattern in the region of Scer\GAL4^ap-md544 expression, in which hairs point in orientations independent of their neighbours. 10% of these flies have wings that show a whorled planar cell polarity. Examination of wing prehair emergence shows that hairs in affected regions initiate aberrantly.