FlyBase Allele Report: Dmel\scf[RNAi.UAS]

General Information

Symbol

Dmel\scf^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0197354

Feature type

allele

Associated gene

Dmel\scf

Associated Insertion(s)

Carried in Construct

P{UAS-scf.RNAi}

Also Known As

UAS-IRscf

Key Links

Transgenic product class

RNAi_reagent

(Furuhashi et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Furuhashi et al., 2006)

Mutagen

in vitro construct

(Furuhashi et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-scf.RNAi}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat of part of the scf gene. The scf repeat sequences are separated from each other by a 150bp spacer fragment derived from the Avic\GFP gene.

(Furuhashi et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

scf

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | male, with Scer\GAL4^Act5C.PI

(Furuhashi et al., 2006)

partially lethal | male, with Scer\GAL4^Act5C.PI

(Ogasawara et al., 2007)

viable | female, with Scer\GAL4^Act5C.PI

(Ogasawara et al., 2007)

visible | dominant | homeotic | male, with Scer\GAL4^Act5C.PI

(Ogasawara et al., 2007)

Phenotype Manifest In

adult abdominal segment 5 | ectopic | male, with Scer\GAL4^Act5C.PI

(Ogasawara et al., 2007)

adult abdominal segment 6 | male, with Scer\GAL4^Act5C.PI

(Ogasawara et al., 2007)

Detailed Description

Statement

Reference

Escaper male adults expressing scf^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PI have ectopic bristles on the ventral side of segment A6, indicating anterior transformation (A6 to A5).

Females expressing scf^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PI are viable.

(Ogasawara et al., 2007)

Expression of scf^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PI results in a dramatic reduction in male viability; most males die during the pupal stage, and those that do survive to adulthood die shortly after eclosion. Expression of scf^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PI has a variable and less pronounced effect on female viability.

(Furuhashi et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Abd-B^HCJ199/Abd-B[+], Scer\GAL4^Act5C.PI, scf^RNAi.UAS has lethal | female phenotype

(Ogasawara et al., 2007)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The viability of females expressing scf^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PI in a Abd-B^HCJ199/+ background is one-tenth that of wild-type females.

(Ogasawara et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

scf^RNAi.UAS

scf^{dsRNA.Scer\UAS}

scf^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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