FB2026_02 , released June 18, 2026
Allele: Dmel\CkIαRNAi.CRL.UAS
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General Information
Symbol
Dmel\CkIαRNAi.CRL.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0197557
Feature type
allele
Associated gene
Dmel\CkIα
Associated Insertion(s)
Carried in Construct
P{UAS-CkIα.CRL.RNAi}
Also Known As
CRL
Key Links
Transgenic product class
RNAi_reagent
(Jia et al., 2005)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Jia et al., 2005)
Mutagen
in vitro construct
(Jia et al., 2005)
Progenitor genotype
Carried in construct
P{UAS-CkIα.CRL.RNAi}
Cytology
Description

UASt sequences drive expression of a genomic fragment encoding amino acids 11--337 of the CkIα gene with the corresponding cDNA fragment inserted in a reverse orientation. The dsRNA produced knocks down expression of CkIα and also of dco (the dsRNA fragment includes a 30bp contiguous sequence that is shared between CkIα and dco).

(Jia et al., 2005)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
CkIα
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Transcription from the P{UAS-CkIα.CRL.dsRNA} construct should produce CkIα dsRNA, resulting in dsRNA interference (RNAi) of the CkIα gene.

      (Jia et al., 2005)

      The P{UAS-CkIα.CRL.dsRNA} construct provides a more efficient knockdown of CkIα than the P{UAS-CkIα.RNAi} construct.

      (Jia et al., 2005)

      This construct results in an effective knockdown of the CkIα gene, but also results in a partial knockdown of the dco gene.

      (Zhang et al., 2006)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      CkIαRNAi.CRL.UAS
      CkIαdsRNA.CRL.Scer\UAS
      CkIαdsRNA.CRL.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (5)