UASt sequences drive expression of a genomic fragment encoding amino acids 11--337 of the CkIα gene with the corresponding cDNA fragment inserted in a reverse orientation. The dsRNA produced knocks down expression of CkIα and also of dco (the dsRNA fragment includes a 30bp contiguous sequence that is shared between CkIα and dco).
UAS sequences drive expression of a genomic fragment encoding amino acids 11--337 of the CkIα gene with the corresponding cDNA fragment inserted in a reverse orientation. The dsRNA produced knocks down expression of CkIα and also of dco (the dsRNA fragment includes a 30bp contiguous sequence that is shared between CkIα and dco).