FlyBase Allele Report: Dmel\SPE[active.UAS.Tag:V5]

General Information

Symbol

Dmel\SPE^{active.UAS.Tag:V5}

Species

D. melanogaster

Name

FlyBase ID

FBal0197590

Feature type

allele

Associated gene

Dmel\SPE

Associated Insertion(s)

Carried in Construct

P{UAS-SPE.a}

Also Known As

UAS-aSPE

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Jang et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-SPE.a}

Cytology

Description

UASt regulatory sequences drive expression of an active form of SPE which does not require post-translational processing. This is achieved by fusing a signal peptide (Tag:SS(ea)) to the SPE catalytic domain (amino acids 135-400). The protein is also tagged with Tag:V5.

(Jang et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Tagged with

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response, with Scer\GAL4^76B

(Paddibhatla et al., 2010)

abnormal immune response, with Scer\GAL4^Cg.PA

(Paddibhatla et al., 2010)

abnormal immune response, with Scer\GAL4^Hml.PG

(Paddibhatla et al., 2010)

abnormal immune response, with Scer\GAL4^Lsp2.PH

(Paddibhatla et al., 2010)

Phenotype Manifest In

embryonic/larval fat body, with Scer\GAL4^76B

(Paddibhatla et al., 2010)

embryonic/larval hemocoel, with Scer\GAL4^76B

(Paddibhatla et al., 2010)

Detailed Description

Statement

Reference

Misexpression of SPE^{active.Scer\UAS.T:SV5\V5} either in the fat body (Scer\GAL4^Lsp2.PH), or hematopoietic (Scer\GAL4^Hml.PG) or both (Scer\GAL4^Cg.PA) induces cell aggregation and melanization upon L.victoriae parasitic wasp infection.

Expression of SPE^{active.Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^76B significantly increases the infiltration index of blood cells into the fat body, indicating an increase in inflammatory immune response.

(Paddibhatla et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^Act5C.PI/SPE^{active.UAS.Tag:V5} is a suppressor of abnormal immune response | recessive phenotype of SPE^Pasteur

(El Chamy et al., 2008)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

The SPE^Pasteur immune-deficiency phenotype can be rescued by expression of SPE^{active.Scer\UAS.T:SV5\V5} driven byScer\GAL4^Act5C.PI.

(El Chamy et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

SPE^*.UAS

(Fullaondo et al., 2011)

SPE^{active.Scer\UAS.T:SV5\V5}

SPE^{active.UAS.Tag:V5}

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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