FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\ProtBEGFP
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General Information
Symbol
Dmel\ProtBEGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0197722
Feature type
allele
Associated gene
Dmel\ProtB
Associated Insertion(s)
Carried in Construct
P{protamineB-eGFP}
Also Known As
ProtB-GFP, protamineB-eGFP, ProtB-eGFP, Protamine-GFP
Key Links
Transgenic product class
wild_type
(Raja and Renkawitz-Pohl, 2005)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Raja and Renkawitz-Pohl, 2005)
Mutagen
in vitro construct
(Raja and Renkawitz-Pohl, 2005)
Progenitor genotype
Carried in construct
P{protamineB-eGFP}
Cytology
Description

ProtB is expressed from its own promoter and is tagged with EGFP in frame with the last ProtB exon. ProtB sequences correspond to 665bp upstream of the predicted 5' UTR to the last exon.

(Raja and Renkawitz-Pohl, 2005)
Allele components
Component
Use(s)
Regulatory region(s)
ProtB
Encoded product / tool
ProtB
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter or direct label
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      spermatogenesis & adult stage | male
      spermatozoon
      • nucleus
      (Minami et al., 2012)
      Additional Information
      Statement
      Reference
       
      Marker for
      spermatozoon
      (Minami et al., 2012)
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      One copy of ProtBT:Avic\GFP-EGFP leads to no obvious spermatogenesis defects,as compared to controls.

      (Kimura and Loppin, 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT Suppressor of
      Statement
      Reference

      ProtB[+]/ProtBEGFP is a non-suppressor of spermatid cyst | spermatogenesis phenotype of Mst77FΔ1

      (Kimura and Loppin, 2016)

      ProtB[+]/ProtBEGFP is a non-suppressor of individualization stage spermatid | spermatogenesis phenotype of Mst77FΔ1

      (Kimura and Loppin, 2016)

      ProtB[+]/ProtBEGFP is a non-suppressor of cystic bulge | spermatogenesis phenotype of Mst77FΔ1

      (Kimura and Loppin, 2016)

      ProtB[+]/ProtBEGFP is a non-suppressor of nucleus | spermatogenesis phenotype of Mst77FΔ1

      (Kimura and Loppin, 2016)

      ProtB[+]/ProtBEGFP is a non-suppressor of chromatin | spermatogenesis phenotype of Mst77FΔ1

      (Kimura and Loppin, 2016)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      One copy of ProtBT:Avic\GFP-EGFP does not suppress the spermatogenesis defects exhibited by Mst77FΔ1 homozygotes.

      (Kimura and Loppin, 2016)

      Expression of Mst35BbT:Avic\GFP-EGFP fails to suppress the spermatid phenotype of Mst77F1/Df(3L)ri-79c males.

      (Raja and Renkawitz-Pohl, 2005)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      ProtBEGFP rescues Df(2L)FDD-0338164

      (Rathke et al., 2010)
      Comments

      One copy of Mst35BbT:Avic\GFP-EGFP reduces the number of malformed sperm heads seen in Df(2L)FDD-0338164 males, while two copies of Mst35BbT:Avic\GFP-EGFP completely rescue this phenotype.

      (Rathke et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (3)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Mst35BbT:Avic\GFP-EGFP
      ProtBEGFP
      ProtBT:Avic\GFP-EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (25)