FlyBase Recombinant Construct Report: P{protamineB-eGFP}

General Information

Symbol

P{protamineB-eGFP}

FlyBase ID

FBtp0023348

Feature type

transgenic_transposable_element

Component Allele(s)

ProtB^EGFP

Also Known As

ProtB-GFP, protamineB-eGFP, ProtB-eGFP, Protamine-GFP

Size

Expression Data

Associated insertion(s)

2 available

Molecular map

Description and Uses

Construct components

Component allele ProtB^EGFP

Transgenic product class

wild_type

(Raja and Renkawitz-Pohl, 2005)

Component

Use(s)

Regulatory region(s)

ProtB

Encoded product / tool

ProtB

Tagged with

EGFP

green fluorescent protein

Description

ProtB is expressed from its own promoter and is tagged with EGFP in frame with the last ProtB exon. ProtB sequences correspond to 665bp upstream of the predicted 5' UTR to the last exon.

(Raja and Renkawitz-Pohl, 2005)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

ProtB^EGFP

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter or direct label

Stage

Tissue/Position (including subcellular localization)

Reference

spermatogenesis & adult stage | male

spermatozoon

• nucleus

(Minami et al., 2012)

Additional Information

Statement

Reference

Marker for

spermatozoon

(Minami et al., 2012)

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (3)

Stocks containing insertion(s) of this recombinant construct

Bloomington

58406

w^*; P{protamineB-eGFP}2/CyO; P{dj-GFP.S}3/TM3, Sb¹

58447

w^*; TI{TI}Pkd2^ko67, P{protamineB-eGFP}2/CyO; P{dj-GFP.S}3

58408

w^*; P{protamineB-eGFP}2; P{EPgy2}mld^EY12290 VhaPPA1-2^A144/TM3, Sb¹

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (16)

Reported As

Symbol Synonym

Mst35Bb-EGFP

(Kimura and Loppin, 2016)

Mst35Bb-GFP

(Konev et al., 2007)

ProtB-EGFP

(Meng and Yamashita, 2025)

ProtB-GFP

(Netherton et al., 2024, Dubruille et al., 2023, Iki et al., 2023, Palacios et al., 2021, Iki et al., 2020, Prudêncio et al., 2018, Bonnefoy et al., 2007)

ProtB-eGFP

(Kimura, 2013, Leser et al., 2012)

Protamine B-eGFP

(Vardanyan et al., 2008)

Protamine-GFP

(Yang and Lu, 2011, Yang et al., 2011)

ProtamineB-GFP

(Netherton et al., 2024)

ProtamineB-eGFP

(Iki et al., 2020, Awe and Renkawitz-Pohl, 2010, Raja and Renkawitz-Pohl, 2006)

P{protamineB-eGFP}

(FlyBase, 1992-)

prot-B-eGFP

(Rathke et al., 2010)

protB-GFP

(Butsch et al., 2023)

protamine B-GFP

(Couderc et al., 2017)

protamine-eGFP

(Rathke et al., 2007)

protamineB-eGFP

(Liu et al., 2024, Linnemannstöns et al., 2014, Raja and Renkawitz-Pohl, 2005)

protamineB::GFP

(Minami et al., 2012)

Secondary FlyBase IDs

References (28)

Report Sections

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