FB2026_02 , released June 18, 2026
Allele: Dmel\mfrZ0695
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General Information
Symbol
Dmel\mfrZ0695
Species
D. melanogaster
Name
FlyBase ID
FBal0197732
Feature type
allele
Associated gene
Dmel\mfr
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Smith, 2007)
Progenitor genotype
Cytology
Description

The coordinate for the nucleotide substitution is the genomic sequence location.

(Smith, 2007)

Nucleotide substitution: G8899732A.

(Smith, 2007)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3L:8,906,632..8,906,632 [-]
Nucleotide change:

G8906632A

Reported nucleotide change:

G8899732A

Amino acid change:

W941term | mfr-PB; W1276term | mfr-PC; W1188term | mfr-PD; W1484term | mfr-PF; W1276term | mfr-PI; W485term | mfr-PJ

Reported amino acid change:

W1522term

Comment:

The mutations were reported relative to the longest mfr protein isoform, which uses an upstream ATG start relative to the annotated version in release 5.54.

(Smith, 2007)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      In crosses of mfrZ0695/Df(3L)h-i22 males to wild-type females, there are 86% inseminated eggs compared to crosses performed with control males. Entry of mfrZ0695/Df(3L)h-i22 sperm into the egg, including the sperm tail, happens completely. However, the sperm does not undergo nuclear condensation and membrane markers show that the plasma membrane breakdown that should happen during fertilization does not occur in mfrZ0695/Df(3L)h-i22 sperm.

      14% of eggs laid by mfrZ0695/Df(3L)h-i22 females produce are abnormal. The defects are visible in late stage eggs as abnormally short and closely apposed dorsal appendages, signifying a ventralized egg chamber. mfrZ0695/mfrZ4070 and mfrZ0695/mfrZ1386 females also produce defective eggs with a penetrance of approximately 35%.

      (Smith, 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      mfrZ0695/Df(3L)hry-i22 is rescued by mfr+t10.7

      (Smith, 2007)
      Partially rescued by

      mfrZ0695/mfrZ1386 is partially rescued by mfr+t10.7

      (Smith, 2007)
      Comments

      Expression of one copy of the mfr+t10.7 transgene reduces the penetrance of the defective egg phenotype seen in eggs laid by mfrZ1386/mfrZ0695 females. Expression of two copies effects a complete rescue of the egg phenotype.

      (Smith, 2007)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Selected as: a male sterile mutation from the Zuker collection.

      (Smith, 2007)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      mfrZ0695
      (Smith, 2007)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)