FlyBase Allele Report: Hsap\STUB1[UAS.cAa]

General Information

Symbol

Hsap\STUB1^UAS.cAa

Species

H. sapiens

Name

Saccharomyces cerevisiae UAS construct a of Al-Ramahi

FlyBase ID

FBal0197897

Feature type

allele

Associated gene

Hsap\STUB1

Associated Insertion(s)

Carried in Construct

P{UAS-hSTUB1.A}

Also Known As

UAS-CHIP

Key Links

Transgenic product class

wild_type

(Al-Ramahi et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Al-Ramahi et al., 2006)

Mutagen

in vitro construct

(Al-Ramahi et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-hSTUB1.A}

Cytology

Description

UASt regulatory sequences drive expression of Hsap\STUB1.

(Al-Ramahi et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\STUB1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN1^30Q.UAS

(Al-Ramahi et al., 2006)

modeled by Hsap\ATXN1^2Q.UAS

(Al-Ramahi et al., 2006)

modeled by Hsap\ATXN1^82Q.UAS

(Al-Ramahi et al., 2006)

ameliorates Huntington's disease

modeled by Hsap\HTT^NT.128Q.UAS

(Al-Ramahi et al., 2006)

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Branco et al., 2008)

exacerbates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Branco et al., 2008)

ameliorates olivopontocerebellar atrophy

modeled by Hsap\ATXN1^82Q.UAS

(Branco et al., 2008)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

Hsap\STUB1^Scer\UAS.cAa has different effects on different aspects of the Hsap\HTT^{128Q.1-336.Scer\UAS} Huntington's disease model ; the retinal phenotypes are less severe, but the number of nuclear inclusions is exacerbated.

(Branco et al., 2008)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Hsap\STUB1^UAS.cAa, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Al-Ramahi et al., 2006)

Hsap\STUB1^UAS.cAa, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Hsap\ATXN1^30Q.UAS, Scer\GAL4^GMR.PF

(Al-Ramahi et al., 2006)

Hsap\STUB1^UAS.cAa, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Hsap\ATXN1^2Q.UAS, Scer\GAL4^GMR.PF

(Al-Ramahi et al., 2006)

Hsap\STUB1^UAS.cAa, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Al-Ramahi et al., 2006)

Phenotype Manifest In

Suppressor of

Statement

Reference

Hsap\STUB1^UAS.cAa, Scer\GAL4^GMR.PU is a suppressor of retina phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PU

(Branco et al., 2008)

Hsap\STUB1^UAS.cAa, Scer\GAL4^GMR.PU is a suppressor of retina phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^GMR.PU

(Branco et al., 2008)

Hsap\STUB1^UAS.cAa, Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Hsap\ATXN1^30Q.UAS, Scer\GAL4^GMR.PF

(Al-Ramahi et al., 2006)

Hsap\STUB1^UAS.cAa, Scer\GAL4^GMR.PF is a suppressor of retina phenotype of Hsap\ATXN1^30Q.UAS, Scer\GAL4^GMR.PF

(Al-Ramahi et al., 2006)

Hsap\STUB1^UAS.cAa, Scer\GAL4^GMR.PF is a suppressor of retina phenotype of Hsap\HTT^NT.128Q.UAS, Scer\GAL4^GMR.PF

(Al-Ramahi et al., 2006)

Hsap\STUB1^UAS.cAa, Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Hsap\ATXN1^2Q.UAS, Scer\GAL4^GMR.PF

(Al-Ramahi et al., 2006)

Hsap\STUB1^UAS.cAa, Scer\GAL4^GMR.PF is a suppressor of interommatidial bristle phenotype of Hsap\ATXN1^2Q.UAS, Scer\GAL4^GMR.PF

(Al-Ramahi et al., 2006)

Hsap\STUB1^UAS.cAa, Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Al-Ramahi et al., 2006)

Hsap\STUB1^UAS.cAa, Scer\GAL4^GMR.PF is a suppressor of retina phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Al-Ramahi et al., 2006)

NOT Suppressor of

Statement

Reference

Hsap\STUB1^UAS.cAa, Scer\GAL4^GMR.PF is a non-suppressor of ommatidium phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Al-Ramahi et al., 2006)

Hsap\STUB1^UAS.cAa, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Al-Ramahi et al., 2006)

Hsap\STUB1^UAS.cAa, Scer\GAL4^GMR.PF is a non-suppressor of retina phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Al-Ramahi et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\STUB1^Scer\UAS.cAa suppresses the retinal phenotype seen when Hsap\HTT^{128Q.1-336.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PU.

Expression of Hsap\STUB1^Scer\UAS.cAa suppresses the retinal phenotype seen when Hsap\ATXN1^82Q.Scer\UAS is expressed under the control of Scer\GAL4^GMR.PU.

Expression of Hsap\STUB1^Scer\UAS.cAa reduces the number of neurons that contain nuclear inclusions seen when Hsap\ATXN1^82Q.Scer\UAS is expressed under the control of Scer\GAL4^ey-OK107.

Expression of Hsap\STUB1^Scer\UAS.cAa dramatically increases the number of neurons that contain nuclear inclusions seen when Hsap\HTT^{128Q.1-336.Scer\UAS} is expressed under the control of Scer\GAL4^ey-OK107.

(Branco et al., 2008)

Co-expression of Hsap\STUB1^Scer\UAS.cAa suppresses the eye phenotype caused by expression of Hsap\ATX1^82Q.Scer\UAS under the control of Scer\GAL4^GMR.PF, with the degree of suppression depending on the level of Hsap\STUB1^Scer\UAS.cAa expression.

Co-expression of Hsap\STUB1^Scer\UAS.cAa suppresses the eye phenotype caused by expression of Hsap\ATX1^30Q.Scer\UAS under the control of Scer\GAL4^GMR.PF.

Co-expression of Hsap\STUB1^Scer\UAS.cAa suppresses the eye phenotype caused by expression of Hsap\ATX1^2Q.Scer\UAS under the control of Scer\GAL4^GMR.PF.

Co-expression of Hsap\STUB1^Scer\UAS.cAa does not suppress the eye phenotypes caused by expression of Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PF.

Co-expression of Hsap\STUB1^Scer\UAS.cAa suppresses the retina defects caused by expression of Hsap\HD^{NT.128Q.Scer\UAS} under the control of Scer\GAL4^GMR.PF.

(Al-Ramahi et al., 2006)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hsap\STUB1^Scer\UAS.cAa

Hsap\STUB1^UAS.cAa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Al-Ramahi

Secondary FlyBase IDs

References (2)

Report Sections

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