Expression of egg[dsRNA.Scer\UAS] driven by Scer\GAL4lz-gal4 results in defective eye development, as evidenced by the presence of misshaped and fused ommatidia and lack of bristles.
Expression of eggdsRNA.Scer\UAS driven by Scer\GAL4lz-gal4 results in the attenuation of mitosis in regions posterior to the morphogenetic furrow in eye imaginal discs.
Larvae that express of eggdsRNA.Scer\UAS under the control of Scer\GAL4da.G32 arrest at or just after the molt from first to second larval instar. After the first larval molt these larvae leave the food and migrate up the walls of the vial but do not form puparia and still react to touch 36h after leaving food. The penetrance of this phenotype is 100%.
Expression of eggdsRNA.Scer\UAS in the third larval instar CNS, driven by Scer\GAL4c698a, allows normal development up to and through pupariation, but never results in the eclosion of mature imagos. On pupariation, larvae expressing eggdsRNA.Scer\UAS form bent pupa with an unusual V-shaped anterior end. Pupal head eversion fails to occur properly and melanization of the pupae is not complete. The posterior part is somewhat more tanned than the anterior.
Expression of eggdsRNA.Scer\UAS in the wing disc, driven by Scer\GAL4ap-md544, results in the formation of a "balloon wing" as the entire wing shows blistering. The blisters are initially filled with air and hemolymph and deflate after a while, leaving a clearly visible scar that may cause the wing to bend up.
Scer\GAL4fkh.PH, eggRNAi.UAS.cSa, p53UAS.A.Tag:MYC has increased cell death phenotype
Scer\GAL4fkh.PH, eggRNAi.UAS.cSa, p53UAS.A.Tag:MYC has salivary gland phenotype
Co-expression of eggdsRNA.Scer\UAS and p53Scer\UAS.A.T:Hsap\MYC under the control of Scer\GAL4fkh.PH results in small salivary glands and high levels of apoptotic cell death.
Transcription from the P{UAS-Eset.IR} construct should produce egg dsRNA, resulting in dsRNA interference (RNAi) of the egg gene.