FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\eggRNAi.UAS.cSa
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General Information
Symbol
Dmel\eggRNAi.UAS.cSa
Species
D. melanogaster
Name
FlyBase ID
FBal0198121
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Transgenic product class
Nature of the Allele
Transgenic product class
Progenitor genotype
Carried in construct
Cytology
Description

UAS regulatory sequences drive expression of a 540bp inverted repeat corresponding to a region of egg cDNA. The egg sequences are separated by an H intron.

Allele components
Component
Use(s)
Encoded product / tool
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of egg[dsRNA.Scer\UAS] driven by Scer\GAL4lz-gal4 results in defective eye development, as evidenced by the presence of misshaped and fused ommatidia and lack of bristles.

Expression of eggdsRNA.Scer\UAS driven by Scer\GAL4lz-gal4 results in the attenuation of mitosis in regions posterior to the morphogenetic furrow in eye imaginal discs.

Larvae that express of eggdsRNA.Scer\UAS under the control of Scer\GAL4da.G32 arrest at or just after the molt from first to second larval instar. After the first larval molt these larvae leave the food and migrate up the walls of the vial but do not form puparia and still react to touch 36h after leaving food. The penetrance of this phenotype is 100%.

Expression of eggdsRNA.Scer\UAS in the third larval instar CNS, driven by Scer\GAL4c698a, allows normal development up to and through pupariation, but never results in the eclosion of mature imagos. On pupariation, larvae expressing eggdsRNA.Scer\UAS form bent pupa with an unusual V-shaped anterior end. Pupal head eversion fails to occur properly and melanization of the pupae is not complete. The posterior part is somewhat more tanned than the anterior.

Expression of eggdsRNA.Scer\UAS in the wing disc, driven by Scer\GAL4ap-md544, results in the formation of a "balloon wing" as the entire wing shows blistering. The blisters are initially filled with air and hemolymph and deflate after a while, leaving a clearly visible scar that may cause the wing to bend up.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of eggdsRNA.Scer\UAS and p53Scer\UAS.A.T:Hsap\MYC under the control of Scer\GAL4fkh.PH results in small salivary glands and high levels of apoptotic cell death.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Transcription from the P{UAS-Eset.IR} construct should produce egg dsRNA, resulting in dsRNA interference (RNAi) of the egg gene.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
eggRNAi.UAS.cSa
eggdsRNA.Scer\UAS
eggdsRNA.UAS
Name Synonyms
Secondary FlyBase IDs
    References (5)