FlyBase Allele Report: Dmel\trp[14]

General Information

Symbol

Dmel\trp¹⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0198161

Feature type

allele

Associated gene

Dmel\trp

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Wang et al., 2005)

Progenitor genotype

Cytology

Description

Amino acid replacement: L612F.

(Wang et al., 2005)

Amino acid replacement: R671N.

(Wang et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

MNV

3R:29,917,111..29,917,113 [-]

Reported amino acid change:

R671N

Comment:

The reported Arg671 to Asn amino acid change (CGA to AAT/C) requires substitution at more than one nucleotide position in the codon.

(Wang et al., 2005)

point_mutation

3R:29,917,290..29,917,290 [-]

Nucleotide change:

C29917290T

Amino acid change:

L612F | trp-PA

Reported amino acid change:

L612F

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Wang et al., 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Wang et al., 2005)

abnormal neurophysiology

(Wang et al., 2005)

Phenotype Manifest In

rhabdomere

(Wang et al., 2005)

Detailed Description

Statement

Reference

Mutant flies have electroretinogram defects, although the decline in light response is slower than that seen for strong trp alleles (60% deactivation time for trp¹⁴ flies is 5.2 +/- 1.6 seconds compared to 1.5 +/- 0.2 seconds for trp⁹ flies).

Mutant flies show light-dependent degeneration of rhabdomeres; when maintained under a 12 hour light-12 hour dark cycle rhabdomeres begin to disappear between 7-10 days after eclosion, and almost no rhabdomeres remain after 14 days. In contrast, when maintained in the dark, the number of rhabdomeres per ommatidium does not decrease, even 30 days after eclosion, and the rhabdomeres are normal in size in 30 day old mutant flies.

(Wang et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

trp¹⁴ has abnormal neuroanatomy phenotype, suppressible by Calx^B

(Wang et al., 2005)

trp¹⁴ has abnormal neuroanatomy phenotype, suppressible | partially by Arr2⁵

(Wang et al., 2005)

Suppressor of

Statement

Reference

trp¹⁴ is a suppressor of abnormal neuroanatomy phenotype of Calx^B

(Wang et al., 2005)

Phenotype Manifest In

Suppressed by

Statement

Reference

trp¹⁴ has rhabdomere phenotype, suppressible by Calx^B

(Wang et al., 2005)

trp¹⁴ has rhabdomere phenotype, suppressible | partially by Arr2⁵

(Wang et al., 2005)

Suppressor of

Statement

Reference

trp¹⁴ is a suppressor of rhabdomere phenotype of Calx^B

(Wang et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

trpl³⁰² ; trp¹⁴ flies show a electroretinogram response in response to a 10 second pulse of light that is similar to that seen in trp¹⁴ single mutants.

The retinal degeneration seen in both Calx^B and trp¹⁴ single mutant flies when they are maintained under a 12 hour light-12 hour dark cycle is suppressed in Calx^B trp¹⁴ double mutants; most ommatidia in the double mutants contain the normal number of rhabdomeres at 14 days after eclosion.

Arr2⁵ partially suppresses the retinal degeneration seen in trp¹⁴ single mutant flies when they are maintained under a 12 hour light-12 hour dark cycle; the double mutants contain the normal number of rhabdomeres at 14 days after eclosion, although the size of the rhabdomeres is reduced compared to controls.

(Wang et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

trp⁹

(Wang et al., 2005)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

24498

w^*; sna^Sco/CyO; trp¹⁴

Notes on Origin

Discoverer

Selected as: A mutation on the 3rd chromosome that has electroretinogram defects.

(Wang et al., 2005)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

trp¹⁴

(Voolstra et al., 2017, Richter et al., 2011, Wang et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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