FlyBase Allele Report: Dmel\Act5C[GD1351]

General Information

Symbol

Dmel\Act5C^GD1351

Species

D. melanogaster

Name

FlyBase ID

FBal0198667

Feature type

allele

Associated gene

Dmel\Act5C

Associated Insertion(s)

Carried in Construct

P{GD1351}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1351}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1351

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

sarcomere, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Z disc, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

Detailed Description

Statement

Reference

Knockdown of Act5C through expression of Act5C^GD1351 under the control of Scer\GAL4^dpp.blk1 does not affect the number of campaniform sensilla.

(Hsiao et al., 2014)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in early larval lethality or embryonic lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal larval body wall muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in sarcomeres with discontinuous Z-lines in the larval body wall muscles.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 10-20% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 10-20% of the Scer\GAL4^pnr-MD237 expression domain.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1351

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

Act5C^GD1351/Scer\GAL4^dpp.blk1 is an enhancer of wing sensillum phenotype of sc^10-1

(Hsiao et al., 2014)

Act5C^GD1351/Scer\GAL4^pnr-MD237 is an enhancer of macrochaeta phenotype of sc^10-1

(Hsiao et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Knockdown of Act5C through expression of Act5C^GD1351 under the control of Scer\GAL4^dpp.blk1 results in a dramatic increase in the percentage of sc^10-1 flies missing campaniform sensilla. Likewise, knockdown of Act5C^GD1351 by Scer\GAL4^pnr-MD237 in the sc^10-1/+ fly notum markedly increased the percentage of flies missing anterior dorso-central mechano-sensory macrochaetae from 30% to 87%, and missing posterior dorso-central macrochaetae from 0% to 24%.

(Hsiao et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v7139

w¹¹¹⁸; P{GD1351}v7139

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1351

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Act5C^GD1351

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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