FlyBase Allele Report: Dmel\Appl[GD3170]

General Information

Symbol

Dmel\Appl^GD3170

Species

D. melanogaster

Name

FlyBase ID

FBal0198740

Feature type

allele

Associated gene

Dmel\Appl

Associated Insertion(s)

Carried in Construct

P{GD3170}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD3170}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD3170

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Alzheimer's disease 1

CEA

(Silva et al., 2020)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory | adult stage, with Scer\GAL4^VT064246

(Turrel et al., 2017)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

dense core granule | male limited, with Scer\GAL4^dsx.KI

(Singh et al., 2025)

male accessory gland secondary cell, with Scer\GAL4^dsx.KI

(Singh et al., 2025)

Detailed Description

Statement

Reference

Expression of Appl^GD3170 under the control of Scer\GAL4^VT064246 (using tub-Gal80[ts] to limit the expression to adulthood) results in significantly reduced middle- as well as long-term memory compared to controls (tested in an aversive olfactory memory assay 2hr and 24hr post-training, respectively), but their learning performance is not affected.

(Turrel et al., 2017)

After massed conditioning comprising five consecutive repeated cycles of training, scores at 24 hours are not significantly different when Appl^{G3.dsRNA.Scer\UAS} is expressed until the control of Scer\GAL4^{Mef2.247.Switch}.

(Goguel et al., 2011)

Adults expressing Appl^GD3170 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development