FlyBase Allele Report: Dmel\Sems[GD5539]

General Information

Symbol

Dmel\Sems^GD5539

Species

D. melanogaster

Name

FlyBase ID

FBal0199079

Feature type

allele

Associated gene

Dmel\Sems

Associated Insertion(s)

Carried in Construct

P{GD5539}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD5539}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD5539

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mating behavior, with Scer\GAL4^Tub.PU

(Laflamme et al., 2012)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

The total number of eggs laid over 10 days is significantly lower in females mated to Sems^GD5539, Scer\GAL4^tub.PU males relative to their controls. (This egg laying defect is not accompanied by a hatchability defect.) Decreased egg laying is only apparent after the first day of mating and persists until at least 9 days after mating.

Females mated to Sems^GD5539, Scer\GAL4^tub.PU males are significantly more likely to remate at 2 days and 4 days after the start of mating.

Females mated to Sems^GD5539, Scer\GAL4^tub.PU males store normal numbers of sperm but significantly more sperm remain in storage at 10 days after the start of mating compared to controls. This effect is due to a failure to release sperm from the seminal receptacle.

(Laflamme et al., 2012)

Adults expressing Sems^GD5539 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development