UASt regulatory sequences drive expression of an inverted repeat.
Ectopic expression of spoonGD3235 RNAi (and UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4nos.PU results in significant reduction of mtDNA replication in mitochondria in female germaria.
Adults expressing spoonGD3235 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.