FlyBase Gene Report: Dmel\spoon

General Information

Symbol

Dmel\spoon

Species

D. melanogaster

Name

spoonbill

Annotation Symbol

CG3249

Feature Type

protein_coding_gene

FlyBase ID

FBgn0263987

Gene Model Status

Current

Stock Availability

13 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Pathway
Interactive Fly

Also Known As

yu, PKA anchor protein, AKAP Yu, mdi

Key Links

Genomic Location

Cytogenetic map

4F9-4F10

Sequence location

X:5,412,386..5,420,867 [-]

Recombination map

1-11

RefSeq locus

NC_004354 REGION:5412386..5420867

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (19 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables JUN kinase binding

inferred from physical interaction with FLYBASE:bsk; FB:FBgn0000229

(Das et al., 2023)

enables mitochondrion-plasma membrane adaptor activity

inferred from genetic interaction with FLYBASE:larp; FB:FBgn0261618

(Zhang et al., 2016)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables molecular adaptor activity

inferred from sequence or structural similarity with UniProtKB:O88884

(Gene Ontology Curators, 2002-)

enables nucleic acid binding

inferred from electronic annotation with InterPro:IPR004087

(InterPro Project Members, 2004-)

enables RNA binding

inferred from electronic annotation with InterPro:IPR004088, InterPro:IPR036612

(InterPro Project Members, 2004-)

Biological Process (12 terms)

Terms Based on Experimental Evidence (12 terms)

CV Term

Evidence

References

involved_in border follicle cell migration

inferred from mutant phenotype

(Neuman-Silberberg, 2007)

involved_in centripetally migrating follicle cell migration

inferred from mutant phenotype

(Neuman-Silberberg, 2007)

involved_in dorsal appendage formation

inferred from mutant phenotype

(Neuman-Silberberg, 2007)

involved_in long-term memory

inferred from mutant phenotype

(Lu et al., 2007)

involved_in maternal determination of dorsal/ventral axis, ovarian follicular epithelium, germ-line encoded

inferred from mutant phenotype

(Neuman-Silberberg, 2007)

involved_in negative regulation of actin filament polymerization

inferred from mutant phenotype

(Neuman-Silberberg, 2007)

inferred from genetic interaction with FLYBASE:qua; FB:FBgn0003187

(Neuman-Silberberg, 2007)

acts_upstream_of positive regulation of apoptotic process

inferred from mutant phenotype with FLYBASE:egr; FB:FBgn0033483

(Das et al., 2023)

involved_in positive regulation of cytoplasmic translation

inferred from mutant phenotype

(Zhang et al., 2019, Zhang et al., 2016)

involved_in positive regulation of JNK cascade

inferred from genetic interaction with FLYBASE:Tak1; FB:FBgn0026323

(Das et al., 2023)

acts_upstream_of positive regulation of protein localization to mitochondrion

inferred from mutant phenotype

(Zhang et al., 2016)

involved_in positive regulation of tumor necrosis factor-mediated signaling pathway

inferred from genetic interaction with FLYBASE:egr; FB:FBgn0033483

(Das et al., 2023)

involved_in thermosensory behavior

inferred from mutant phenotype

(Hong et al., 2008)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

colocalizes_with Golgi apparatus

inferred from direct assay

(Hadad et al., 2011)

is_active_in mitochondrial outer membrane

inferred from direct assay

(Das et al., 2023, Zhang et al., 2019, Zhang et al., 2016, Hadad et al., 2011)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

Pathway (FlyBase)

POSITIVE REGULATORS OF TNFalpha-EIGER SIGNALING PATHWAY

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Pathway (FlyBase)

POSITIVE REGULATORS OF TNFalpha-EIGER SIGNALING PATHWAY -: Positive regulators of Tumor Necrosis Factor α (TNFα) signaling pathway up-regulate the activation of the pathway triggered by Eiger (egr).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\spoon for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q7KVW1)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.50

Gene model reviewed during 5.55

Gene model reviewed during 5.39

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 6.61

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

spoon-RA

FBtr0070794

NM_131993

3341

585

spoon-RC

FBtr0070795

NM_167034

4509

607

spoon-RD

FBtr0308688

NM_167033

3668

585

spoon-RE

FBtr0308689

NM_001258603

3179

585

spoon-RF

FBtr0482680

4546

585

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

spoon-PA

FBpp0070760

64.9

585

6.27

FBpp0070761

67.3

607

6.77

FBpp0300894

64.9

585

6.27

FBpp0300895

64.9

585

6.27

FBpp0428600

64.9

585

6.27

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

585 aa isoforms: spoon-PA, spoon-PD, spoon-PE, spoon-PF

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\spoon using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.54

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | faint | ubiquitous

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

dorsal pharyngeal muscle primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

somatic muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

trunk visceral muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 13 -- 16

dorsal pharyngeal muscle cell

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

larval muscle system

(Fisher et al., 2012)

larval somatic muscle cell

(Fisher et al., 2012)

oogenesis

ovary

Comment: transcript A

(Hadad et al., 2011)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- pupal stage

organism

(Hadad et al., 2011)

Additional Descriptive Data

spoon-RA transcipts are most abundant in the ovary. THey are already detectable in the nurse cell cytoplasm of the youngest egg chambers and peak at oogenesis stages 9-10. Other isoforms are present at very low levels.

(Hadad et al., 2011)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

(Lu et al., 2007)

(Hadad et al., 2011)

germarium region 3

(Hadad et al., 2011)

oogenesis stage S1 -- S9

nurse cell

(Hadad et al., 2011)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

spoon protein is expressed early in oogenesis (from stage 2 of the germarium) and persists in nurse cells until stage S9.

(Hadad et al., 2011)

Marker for

Subcellular Localization

CV Term

Evidence

References

colocalizes_with Golgi apparatus

inferred from direct assay

(Hadad et al., 2011)

is_active_in mitochondrial outer membrane

inferred from direct assay

(Das et al., 2023, Zhang et al., 2019, Zhang et al., 2016, Hadad et al., 2011)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\spoon in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\spoon

View exonic expression by tissue for Dmel\spoon

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0263987

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31459/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0263987

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0029764

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0263987

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3249

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 20 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 17 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of spoon

Transgenic constructs containing regulatory region of spoon

Aberrations (Deficiencies and Duplications) ( 11 )

Inferred from experimentation ( 11 )

Gene disrupted in

Df(1)BA2-8

(Hadad et al., 2011)

Df(1)Exel6290

(Hadad et al., 2011, Ryder, 2004.4.26)

Df(1)HC244

(Hadad et al., 2011)

Df(1)ovo41

(Hadad et al., 2011)

Gene not disrupted in

Df(1)bi-D2

(Hadad et al., 2011)

Df(1)C149

(Hadad et al., 2011)

Df(1)Exel6234

(Hadad et al., 2011)

Df(1)Exel6235

(Hadad et al., 2011)

Df(1)JC70

(Hadad et al., 2011)

Df(1)ovo4

(Hadad et al., 2011)

Df(1)RC40

(Hadad et al., 2011)

Inferred from location ( 13 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal - majority die | maternal effect

spoon^mdi-1

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

spoon^GD3235

viable, with Scer\GAL4^Mef2.PR

spoon^GD3235

viable, with Scer\GAL4^tin.CΔ4

spoon^GD3235

Sterility

Allele

female semi-sterile | recessive

spoon^mdi-1

female sterile

female sterile germline-dependent, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

spoon^RNAi.UAS.V22

fertile

spoon^EY08737

Other Phenotypes

Allele

abnormal memory (with spoon^KG02745)

spoon^P1

abnormal memory (with spoon^P1)

spoon^KG02745

abnormal memory | recessive

spoon^P1

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav-C155

spoon^EP1400

increased cell death | larval stage, with Scer\GAL4^ptc.PU

spoon^UAS.Tag:HA

increased mortality during development | maternal effect

spoon^mdi-1

majority die during embryonic stage | maternal effect

spoon^mdi-1

Phenotype manifest in

Allele

actin filament & egg chamber

spoon¹

anterior crossvein, with Scer\GAL4^ptc.PU

spoon^UAS.Tag:HA

anterior fascicle & synapse, with Scer\GAL4^elav-C155

spoon^EP1400

border follicle cell

spoon¹

border follicle cell | somatic clone

spoon¹

centripetal follicle cell | germline clone

spoon¹

dorsal appendage | germline clone | maternal effect

spoon¹

dorsal appendage | maternal effect

spoon¹

egg

spoon^mdi-1

egg | maternal effect

spoon^mdi-1

egg chamber

spoon^mdi-1

egg chorion | maternal effect

spoon¹

eye, with Scer\GAL4^GMR.PU

spoon^UAS.Tag:HA

germarium

spoon^mdi-1

germarium, with Scer\GAL4^nanos.PU

spoon^GD3235

larval intersegmental nerve, with Scer\GAL4^elav-C155

spoon^EP1400

mitochondrion | adult stage, with Scer\GAL4^nanos.PU

spoon^GD3235

mitochondrion | maternal effect

spoon^mdi-1

mitochondrion | oogenesis

spoon^mdi-1

nurse cell

spoon¹

nurse cell | germline clone

spoon¹

ommatidium, with Scer\GAL4^GMR.PU

spoon^UAS.Tag:HA

polar follicle cell | somatic clone

spoon¹

wing blade, with Scer\GAL4^ptc.PU

spoon^UAS.Tag:HA

wing disc, with Scer\GAL4^ptc.PU

spoon^UAS.Tag:HA

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (14)

Hsap\AKAP1

10 of 14

Yes

3 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (12)

Rnor\Akap1

9 of 14

Yes

3 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (12)

Mmus\Akap1

10 of 14

Yes

3 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (10)

Xtro\akap1

6 of 13

Yes

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\tdrd15

1 of 13

Danio rerio (Zebrafish) (14)

Drer\akap1a

11 of 14

Yes

Drer\akap1b

9 of 14

Yes

3 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Drer\si:ch211-189e2.3

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\akap-1

9 of 14

Yes

Anopheles gambiae (African malaria mosquito) (7)

Agam\AgaP_AGAP002166

12 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (2)

Atha\Tudor1

1 of 13

Yes

Atha\Tudor2

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:spoon. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (9)

papi

3 of 13

qin

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

tud

1 of 13

vret

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 4 )

Allele

Disease

Interaction

References

spoon^KG02745

ameliorates Parkinson's disease 2

modeled by park¹³

(Das et al., 2023)

exacerbates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN8OS^CTG112.UAS

(Mutsuddi et al., 2004)

spoon^EP1400

ameliorates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN8OS^CTG112.UAS

(Mutsuddi et al., 2004)

exacerbates spinocerebellar ataxia 8

modeled by Hsap\ATXN8OS^CTG112.UAS

(Tripathi et al., 2016)

spoon^UAS.Tag:HA

ameliorates spinocerebellar ataxia 8

modeled by Hsap\ATXN8OS^CTG112.UAS

(Tripathi et al., 2016)

spoon^{KH.UAS.Tag:HA}

ameliorates spinocerebellar ataxia 8

modeled by Hsap\ATXN8OS^CTG112.UAS

(Tripathi et al., 2016)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

AKAP1; A-kinase anchoring protein 1

10 of 14

602449

TDRKH; tudor and KH domain containing

3 of 14

609501

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Dmel gene

Ortholog showing functional complementation

Supporting References

spoon

Hsap\AKAP1

(Zhang et al., 2016)

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

spoon - S6kII

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

spoon - Tsc1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

spoon - bsk

anti tag coimmunoprecipitation, western blot

(Das et al., 2023)

spoon - eIF2A

experimental knowledge based

(Guruharsha et al., 2011)

spoon - larp

anti tag coimmunoprecipitation, western blot, peptide massfingerprinting

(Zhang et al., 2016)

spoon - p53

pull down, autoradiography

(Lunardi et al., 2010)

spoon - pros

anti bait coimmunoprecipitation, western blot

(Tripathi et al., 2017)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Das et al., 2023)

suppressible

(Zhang et al., 2019)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Das et al., 2023)

suppressible

(Das et al., 2023)

suppressible

(Neuman-Silberberg, 2007)

cup

suppressible

spoon

(Neuman-Silberberg, 2007)

suppressible

(Das et al., 2023)

suppressible

(Das et al., 2023)

enhanceable

(Das et al., 2023)

suppressible

(Das et al., 2023)

suppressible

(Das et al., 2023)

suppressible

(Das et al., 2023)

suppressible

(Neuman-Silberberg, 2007)

suppressible

(Das et al., 2023)

suppressible

(Das et al., 2023)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0263987

MIST (protein-protein) - An integrated Molecular Interaction Database

31459

Pathways

Signaling Pathways (FlyBase)

POSITIVE REGULATORS OF TNFalpha-EIGER SIGNALING PATHWAY

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Factors involved in megakaryocyte development and platelet production

Class of Gene

gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-11

Cytogenetic map

4F9-4F10

Sequence location

X:5,412,386..5,420,867 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

4F9-4F10

Limits computationally determined from genome sequence between P{EP}EP425 and P{EP}CG3249^EP1400

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-11

(FlyBase, 2016)

1-13.9

(Comeron et al., 2012)

1-14

(Hadad et al., 2011)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (13)

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (281)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

DPiM_GM14014

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0263987

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Tripathi et al., 2017, Zhang et al., 2016, Hadad et al., 2011)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

yu is required for olfactory long-term memory formation.

(Lu et al., 2007)

spoon is required largely in th germline for the correct patterning of the egg chamber. However, it may also be required in the soma and may play a role in the proper specification of border cell cluster polarity.

(Neuman-Silberberg, 2007)

The spoon gene may function as a positive regulator upstream of both grk/Egfr and the dpp pathway.

(Neuman-Silberberg and Motola, 2003)

Mutants show female sterility. In spoon mutant egg chambers the selective transport of grk RNA from the nurse cells into the oocyte, as well as its proper localization and translation, are disrupted.

(Neuman-Silberberg and Motola, 2003)

Relationship to Other Genes

Source for database merge of

Source for merge of: CG3249 BcDNA:GM04319

(FlyBase, 1996-)

Source for merge of: spoon yu

(Hadad et al., 2011)

Additional comments

Source for merge of CG3249 BcDNA:GM04319 was a shared cDNA ( date:030728 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: yu CG3249

(Lu et al., 2007)

Nomenclature comments

Etymology

The gene is named "mitochondrial DNA insufficient" based on the mutant phenotype of reduced mitochondrial DNA replication.

(Zhang et al., 2016)

Gene is named "yu" (yu means "foolish" in Chinese) as mutants show defects in memory.

(Lu et al., 2007)

Synonyms and Secondary IDs (12)

Reported As

Symbol Synonym

AKAP Yu

(Zhao et al., 2013)

BcDNA:GM04319

CG3249

(Zhang et al., 2016, Brooks et al., 2015, Japanese National Institute of Genetics, 2012.5.21, Hadad et al., 2011, Lunardi et al., 2010, Ni et al., 2010.12.1, Blagden et al., 2009, Keleman et al., 2009.8.5, Chen et al., 2008, Lu et al., 2007, Manh et al., 2005)

PKAAP

(Mutsuddi et al., 2004)

Spoon

(Ge et al., 2019, Tripathi et al., 2017)

(Siomi et al., 2010)

mdi

(Zhang et al., 2019, Zhang et al., 2016)

spoon

(Anqueira-González et al., 2021, Kim et al., 2021, Schwartz et al., 2016, Tripathi et al., 2016, Brooks et al., 2015, Jambor et al., 2015, Olesnicky et al., 2014, Hadad et al., 2011, Neuman-Silberberg, 2007, Motola and Neuman-Silberberg, 2004)

(Duvall and Taghert, 2013, Scheunemann et al., 2013, Duvall and Taghert, 2012, Hadad et al., 2011, Handler et al., 2011, Lu et al., 2007)

Name Synonyms

AKAP Yu

(Zhao et al., 2013)

CG3249

PKA anchor protein

(Hong et al., 2008, Kraut et al., 2001, Kraut et al., 2001)

mitochondrial DNA insufficient

(Zhang et al., 2016)

spoonbill

(Das et al., 2023, Tripathi et al., 2016, Scheunemann et al., 2013, Neuman-Silberberg, 2007, Motola and Neuman-Silberberg, 2004, Neuman-Silberberg and Motola, 2003)

(Lu et al., 2007)

Secondary FlyBase IDs

FBgn0082162
FBgn0029764
FBgn0047280

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 62 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

31459

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q7KVW1

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q7KVW1

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0263987

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31459/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0263987

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0263987

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG3249

MARRVEL_MODEL - MARRVEL (model organism gene)

31459

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0263987

DroID - A comprehensive database of gene and protein interactions.

FBgn0263987

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0029764

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0263987

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3249

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0263987

MIST (protein-protein) - An integrated Molecular Interaction Database

31459

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Factors involved in megakaryocyte development and platelet production

References (65)

The following external sites may use different assemblies or annotations than FlyBase.

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